Proteinase K / PROK Rabbit Polyclonal Antibody

CAT#: AP21385BT-N

Proteinase K / PROK rabbit polyclonal antibody, Biotin

Specifications

Product Data

• Applications: ELISA, ID, IF, IP, R, WB
• Recommended Dilution: This product is intended for use in precipitating and non-precipitating antibody-binding assays (such as e.g., ELISA and Western blotting and immunofluorescence or histochemical techniques).
  Working dilutions: vary widely, but may be between 1/10000 and 1/1000000.
• Host: Rabbit
• Isotype: IgG
• Clonality: Polyclonal
• Immunogen: Proteinase K isolated and purified from Tritirachium album. Freund’s complete adjuvant is used in the first step of the immunization procedure.
• Specificity: Biotin-conjugated IgG fraction of polyclonal rabbit antiserum to proteinase K from Tritirachium album.
  The reagents were evaluated for potency, purity and specificity using most or all of the following techniques: immunoelectrophoresis, cross-immunoelectrophoresis, single radial immunodiffusion (Ouchterlony), block titration, ELISA, immunoblotting and enzyme inhibition.
• Formulation: PBS, pH 7.2 without preservatives and foreign proteins
  Label: Biotin
  State: Lyophilized IgG fraction
  Molar radio: Biotin/ IgG ~6.8
• Reconstitution Method: Restore by adding 1.0 ml of sterile distilled water
• Concentration: lot specific
• Purification: Ammonium Sulphate Precipitation and Ion Exchange Chromatography
• Conjugation: Biotin
• Storage: Store lyophilized at 2-8°C for 6 months or at -20°C long term.
  After reconstitution store the antibody undiluted at 2-8°C for one month or (in aliquots) at -20°C long term.
  Avoid repeated freezing and thawing.
• Stability: Shelf life: one year from despatch.
• Database Link:
  UniProt ID P06873
• Synonyms: Endopeptidase K, Tritirachium alkaline proteinase