Luciferase Rabbit Polyclonal Antibody

CAT#: AP21341AF-N

Luciferase rabbit polyclonal antibody, Azide Free

Specifications

Product Data

• Applications: ELISA, IF, WB
• Recommended Dilution: ELISA.
  Dot blot.
  Immunoblotting.
  Indirect Immunofluorescence.
  Working Dilutions for Non-precipitating antibody-binding techniques: 1/1000-1/10000.
  This product is intended for use in precipitating and non-precipitating antibody-binding assays (such as e.g., ELISA and Western blotting and Immunofluorescence or Histochemical techniques), to prepare an insoluble immuno-affinity adsorbent, for labelling with a marker of choice.
• Reactivities: Photinus pyralis
• Host: Rabbit
• Isotype: IgG
• Clonality: Polyclonal
• Immunogen: Luciferase isolated and purified from Photinus pyralis.
  Freund’s complete adjuvant is used in the first step of the immunization procedure.
• Specificity: IgG fraction of Polyclonal Rabbit antiserum to Luciferase from Photinus pyralis.
  The reagents were evaluated for potency, purity and specificity using most or all of the following techniques: Immunoelectrophoresis, Cross-Immunoelectrophoresis, Single Radial Immunodiffusion (Ouchterlony), block titration, ELISA, Immunoblotting and enzyme inhibition.
  Cross-reactivities against enzymes of other sources may occur but have not been determined.
• Formulation: PBS, pH 7.2 without preservatives and foreign proteins
  State: Azide Free
  State: Lyophilized purified hyperimmune IgG fraction
• Reconstitution Method: Restore by adding 1.0 ml sterile distilled water.
• Concentration: lot specific
• Conjugation: Unconjugated
• Storage: Store lyophilized at 2-8°C for 6 months or at -20°C long term.
  After reconstitution store the antibody undiluted at 2-8°C for one month
  or (in aliquots) at -20°C long term.
  Avoid repeated freezing and thawing.
• Stability: Shelf life: one year from despatch.
• Database Link:
  UniProt ID P08659
• Background: Luciferase from the firefly has become one of the more widely used reporter proteins for the study of gene expression. Luciferase catalyzes a bioluminescent reaction which requires the substrate luciferin as well as Mg2+ and ATP. Mixing these reagents with the cell extract containing luciferase, results in a flash of light that decays rapidly. This light can be detected by a luminometer. The total light emission is proportional to the luciferase activity of the sample.