Isocitrate dehydrogenase / IDH Rabbit Polyclonal Antibody

CAT#: AP21327PU-N

Isocitrate dehydrogenase / IDH rabbit polyclonal antibody, Aff - Purified

Specifications

Product Data

• Applications: ELISA, ID, IF, IP, R, WB
• Recommended Dilution: Immunoprecipiation.
  Can be Used:
  • In precipitating and non-precipitating antibody-binding assays (such as e.g., ELISA and Western blotting and Immunofluorescence or Histochemical techniques).
  • To prepare an insoluble immuno-affinity adsorbent, for labelling with a marker of choice.
  Recommended Working Dilutions:
  Non-precipitating antibody-binding techniques: 1/1,000-1/30,000.
• Reactivities: Porcine
• Host: Rabbit
• Isotype: IgG
• Clonality: Polyclonal
• Immunogen: Isocitrate Dehydrogenase isolated and purified from Porcine heart.
  Freund’s complete adjuvant is used in the first step of the immunization procedure.
• Specificity: Isocitrate Dehydrogenase isolated and purified from Porcine heart.
  The reagents were evaluated for potency, purity and specificity using most or all of the following techniques: Immunoelectrophoresis, Cross-Immunoelectrophoresis, Single Radial Immunodiffusion (Ouchterlony), block titration, ELISA, Immunoblotting and enzyme inhibition.
  Cross-reactivities against enzymes of other sources may occur but have not been determined.
• Formulation: PBS, pH 7.2 stabilized with Dextran, without preservatives and foreign proteins
  State: Aff - Purified
  State: Lyophilized purified hyperimmune IgG fraction
• Reconstitution Method: Restore by adding 0.5 ml sterile distilled water
• Concentration: lot specific
• Purification: Solid Phase Affinity Chromatography
• Conjugation: Unconjugated
• Storage: Store lyophilized at 2-8°C for 6 months or at -20°C long term.
  After reconstitution store the antibody undiluted at 2-8°C for one month
  or (in aliquots) at -20°C long term.
  Avoid repeated freezing and thawing.
• Stability: Shelf life: one year from despatch.
• Database Link:
  UniProt ID P20304
• Background: Isocitrate dehydrogenase is an enzyme involved in the citric acid cycle. It is 416 amino acids long with a molecular weight of approximately 45 kDa.
  Isocitrate dehydrogenase enzymes catalyze the oxidative decarboxylation of isocitrate to produce alfa ketoglutarate. The human genome has 5 IDH genes coding for 3 IDH enzymes. The IDH1 and IDH2 require nicotinamide adeninedinucleotide phosphate (NADP) as co-substrate, whereas IDH3 require nicotinamide adenine dinucleotide (NAD). The IDH2 and 3 are localized in mitochondria and are actively involved in the citric acid cycle (TCA) for energy production in contrast, IDH1 is localized in cytoplasm and peroxisomes where it generates NADPH, reduced form of NADP for biosynthetic and other types of reaction. Since alfa KG and NADPH both are intermediately substrate for a number of cellular process, which allows the possibility of oncogenic or tumor suppressive activities of IDH1.
• Synonyms: PICD, Cytosolic NADP-isocitrate dehydrogenase, ICDH, IDP, Oxalosuccinate decarboxylase