PGP9.5 (UCHL1) (211-221) Goat Polyclonal Antibody

CAT#: AP21292PU-N

PGP9.5 (UCHL1) (211-221) goat polyclonal antibody, Aff - Purified

Product Images

UCHL1 antibody staining of Mouse (A) and Rat (B) Brain lysate at 0.01 ug/ml (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

Specifications

Product Data

• Applications: ELISA, WB
• Recommended Dilution: Peptide ELISA: 1/1000 (Detection Limit).
  Western blot: 0.01-0.03 µg/ml. This antibody detects a band of ~26kDa in Human Brain (Hippocampus) and Mouse and Rat Brain lysates.
  Enzyme immunoassay: This antibody was reported to work in sandwich-type ELISA both as a capturer and as a reporter in combination with AP21291PU-N.
• Reactivities: Bovine, Canine, Human, Porcine, Rat
• Host: Goat
• Clonality: Polyclonal
• Immunogen: Peptide with sequence (aa 211-221) from the C-Terminus of the protein sequence according to NP_004172.2.
• Specificity: Recognizes UCHL1 (aa 211-221).
• Formulation: Tris saline, pH~7.3
  State: Aff - Purified
  State: Liquid purified IgG fraction
  Stabilizer: 0.5% BSA
  Preservative: 0.02% Sodium Azide
• Concentration: lot specific
• Purification: Ammonium Sulphate Precipitation followed by Antigen Affinity Chromatography using the immunizing peptide
• Conjugation: Unconjugated
• Storage: Store undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer.
  Avoid repeated freezing and thawing.
• Stability: Shelf life: one year from despatch.
• Gene Name: ubiquitin C-terminal hydrolase L1
• Database Link:
  Entrez Gene 7345 Human
  UniProt ID P09936
• Background: Protein gene product 9.5 (PGP9.5) is a member of a gene family whose products hydrolyze small C-terminal adducts of ubiquitin to generate the ubiquitin monomer. Expression of PGP9.5 is highly specific to neurons and to cells of the diffuse neuroendocrine system and their tumors. It is present in all neurons.
• Synonyms: UCH-L1, PGP 9.5, Ubiquitin thioesterase L1, Neuron cytoplasmic protein 9.5
• Note: Calculated Molecular Weight: 24.8kDa (Human NP_004172.2, Mouse NP_035800.2 and Rat NP_058933.2)