Butyrylcholinesterase (BCHE) Rabbit Polyclonal Antibody

CAT#: AP21238PU-N

Butyrylcholinesterase (BCHE) rabbit polyclonal antibody, Aff - Purified

Specifications

Product Data

• Applications: ELISA, ID, IF, IP, R, WB
• Recommended Dilution: This product is intended for use in precipitating and non-precipitating antibody-binding assays (such as e.g., ELISA and Western blotting and Immunofluorescence or Histochemical techniques), to prepare an insoluble immuno-affinity adsorbent, for labelling with a marker of the Customer's own choice.
  Working Dilutions in non-precipitating antibody-binding techniques:1/1,000-1/10,000.
• Reactivities: Human
• Host: Rabbit
• Isotype: IgG
• Clonality: Polyclonal
• Immunogen: Butyrylcholinesterase isolated and purified from Human serum.
  Freund’s complete adjuvant is used in the first step of the immunization procedure.
• Specificity: The antibody recognizes Butyrylcholinesterase from Human Serum.
  The reagents were evaluated for potency, purity and specificity using most or all of the following techniques: Immunoelectrophoresis, Cross-Immunoelectrophoresis, single Radial Immunodiffusion (Ouchterlony), block titration, ELISA, Immunoblotting and Enzyme Inhibition.
  Cross-reactivities against enzymes of other sources may occur but have not been determined.
• Formulation: PBS, pH 7.2 stabilized with Dextran, without preservatives and foreign proteins
  State: Aff - Purified
  State: Lyophilized purified hyperimmune IgG fraction
• Reconstitution Method: Restore by adding 0.5 ml of sterile distilled water. Prepare working dilutions by adding sterile phosphate buffered saline (PBS, ph 7.2) and preferably use the same day.
• Concentration: lot specific
• Purification: Solid Phase Affinity Chromatography
• Conjugation: Unconjugated
• Storage: Store lyophilized at 2-8°C for 6 months or at -20°C long term.
  After reconstitution store the antibody undiluted at 2-8°C for one month
  or (in aliquots) at -20°C long term.
  If a slight precipitation occurs upon storage, this should be removed by centrifugation.
  Avoid repeated freezing and thawing.
• Stability: Shelf life: one year from despatch.
• Gene Name: butyrylcholinesterase
• Database Link:
  Entrez Gene 590 Human
  UniProt ID P06276
• Background: Butyrylcholinesterase is synthetizised in the liver, and is predominantly found in serum, liver and pancreas. This enzyme is a tetrameric glycoprotein (molecular mass of 350 kDa), and consists of four subunits. Defects in Butyrylcholinesterase are the cause of a metabolic disorder characterized by prolonged apnoea after the use of certain anesthetic drugs, including the muscle relaxants succinylcholine or mivacurium and other ester local anesthetics.
• Synonyms: Butyrylcholine esterase, Pseudocholinesterase, BCHE, CHE1

Reference Data

• Protein Families: Druggable Genome, Transmembrane