L1CAM Rabbit Polyclonal Antibody

CAT#: AP21093PU-N

L1CAM rabbit polyclonal antibody, Aff - Purified

Product Images

Western blot (WB) analysis of CD171 / L1CAM Antibody at 1/500 dilution in extracts from Raw264.7 cells treated with LPS.

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  Immunohistochemistry analysis of CD171 / L1CAM Antibody in paraffin-embedded human brain tissue.

Specifications

Product Data

• Applications: IF, IHC, WB
• Recommended Dilution: Western blot: 1/500-1/1000.
  Immunofluorescence: 1/50-1/200.
  Immunohistochemistry on Paraffin Sections: 1/50-1/200.
• Reactivities: Human, Mouse, Rat
• Host: Rabbit
• Clonality: Polyclonal
• Immunogen: Synthetic peptide corresponding to amino acids 1150-1200 of Human NCAM-L1.
• Specificity: This antibody detects endogenous levels of NCAM-L1 protein. AP21093PU-N binds to the cytoplasmic region of NCAM-L1.
• Formulation: Phosphate Buffered Saline (PBS), pH~7.2
  State: Aff - Purified
  State: Liquid purified Ig fraction (> 95% by SDS-PAGE)
  Preservative: 15mM Sodium Azide
• Concentration: 1.0 mg/ml
• Purification: Affinity Chromatography using epitope-specific immunogen
• Conjugation: Unconjugated
• Storage: Store undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer.
  Avoid repeated freezing and thawing.
• Stability: Shelf life: one year from despatch.
• Predicted Protein Size: ~140 kDa
• Gene Name: L1 cell adhesion molecule
• Database Link:
  Entrez Gene 16728 MouseEntrez Gene 50687 RatEntrez Gene 3897 Human
  UniProt ID P32004
• Background: The L1CAM gene, which is located in Xq28, is involved in three distinct conditions: 1) HSAS (hydrocephalus-stenosis of the aqueduct of Sylvius); 2) MASA (mental retardation, aphasia, shuffling gait, adductus thumbs); and 3) SPG1 (spastic paraplegia). The L1, neural cell adhesion molecule (L1CAM) also plays an important role in axon growth, fasciculation, neural migration and in mediating neuronal differentiation. Expression of L1 protein is restricted to tissues arising from neuroectoderm.
• Synonyms: N-CAM L1, CAML1, MIC5

Reference Data

• Protein Families: Druggable Genome, ES Cell Differentiation/IPS, Transmembrane
• Protein Pathways: Axon guidance, Cell adhesion molecules (CAMs)