IKB beta (NFKBIB) Rabbit Polyclonal Antibody

CAT#: AP21089PU-N

IKB beta (NFKBIB) rabbit polyclonal antibody, Aff - Purified

Product Images

Western blot (WB) analysis of IkB-β antibody (Cat.-No.: AP21089PU-N) at 1/500 dilution Lane 1:Hela whole cell lysate treated with H2O2 Lane 2:HEK293T whole cell lysate treated with LPS Lane 3:Mouse liver tissue lysate Lane 4:Mouse kidney tissue lysate Lane 5:Rat kidney tissuelysate

Specifications

Product Data

• Applications: WB
• Recommended Dilution: Western blot: 1/500 - 1/1000.
• Reactivities: Human
• Host: Rabbit
• Clonality: Polyclonal
• Specificity: This antibody detects endogenous levels of IkappaB-beta protein.
  (region surrounding Ala13)
• Formulation: Phosphate buffered saline (PBS), pH 7.2
  State: Aff - Purified
  State: Liquid purified Ig fraction
  Preservative: 0.05% sodium azide
• Concentration: 1.0 mg/ml
• Purification: Affinity chromatography (> 95% (by SDS-PAGE)
• Conjugation: Unconjugated
• Storage: Store undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer.
  Avoid repeated freezing and thawing.
• Stability: Shelf life: one year from despatch.
• Predicted Protein Size: ~ 37 kDa
• Gene Name: NFKB inhibitor beta
• Database Link:
  Entrez Gene 4793 Human
  UniProt ID Q15653
• Background: Three major forms of IkappaB-like molecules have been identified and each is characterized by multiple copies of ankyrin repeats. IkappaB-alpha and IkappaB-beta appear to be the major regulatory forms of IkappaB in most cells. These proteins interact with p65 or c-Rel containing forms of NFkappaB and block nuclear import by masking the nuclear localization sequences of NFkappaB. The activation of NFkB involves the inducible phosphorylation and subsequent degradation of IkappaB. Immunoblotting easily detects the hyper-phosphorylated forms of IkappaB-alpha, but not phosphorylated IkappaB-beta.
• Synonyms: I-kappa-B-beta, TRIP9, IkB-B, IkB-beta, IkappaBbeta, TR-interacting protein 9

Reference Data

• Protein Families: Stem cell - Pluripotency, Transcription Factors
• Protein Pathways: Adipocytokine signaling pathway, B cell receptor signaling pathway, Chemokine signaling pathway, Cytosolic DNA-sensing pathway, Neurotrophin signaling pathway, NOD-like receptor signaling pathway, RIG-I-like receptor signaling pathway, T cell receptor signaling pathway