NFKB1 pSer932 Rabbit Polyclonal Antibody

CAT#: AP20850PU-N

NFKB1 pSer932 rabbit polyclonal antibody, Aff - Purified

Product Images

Western blot (WB) analysis of p-NFkB-p105 antibody (Cat.-No.: AP20850PU-N) at 1/500 dilutionLane 1: Hela whole cell lysate treated with LPSLane 2: Raw264.7 whole cell lysate treated with LPSLane 3: NIH-3T3 whole cell lysate treated with LPSLane 4: Rat kidney tissue lysate

Specifications

Product Data

• Applications: WB
• Recommended Dilution: Western blot: 1/500 - /1000.
• Reactivities: Human, Mouse, Rat
• Host: Rabbit
• Clonality: Polyclonal
• Specificity: This antibody detects endogenous levels of NFκB-p105/p50 protein only when phosphorylated at Ser932.
• Formulation: Phosphate buffered saline (PBS), pH 7.2.
  State: Aff - Purified
  State: Liquid purified Ig fraction
  Preservative: 0.05% sodium azide
• Concentration: 1.0 mg/ml
• Purification: Affinity chromatography (> 95% (by SDS-PAGE)
• Conjugation: Unconjugated
• Storage: Store undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer.
  Avoid repeated freezing and thawing.
• Stability: Shelf life: one year from despatch.
• Predicted Protein Size: ~ 105 kDa
• Gene Name: nuclear factor kappa B subunit 1
• Database Link:
  Entrez Gene 18033 MouseEntrez Gene 81736 RatEntrez Gene 4790 Human
  UniProt ID P19838
• Background: Proteins encoded by the v-Rel viral oncogene and its cellular homolog, c-Rel, are members of a family of transcription factors that include the two subunits of the transcription factor NFkappaB (p50 and p65) and the Drosophila maternal morphagen, Dorsal. These proteins share sequence homology over a region of 300 amino acids at their NH2-terminus, the region that contains their DNA binding and dimerization domains. The DNA binding activity of NFκB is activated and rapidly transported from the cytoplasm to the nucleus in cells exposed to mitogens or growth factors. cDNAs encoding precursors for two distinct proteins have been described. These proteins, designated p105 and p100, are highly related but map on different chromosomes. The p105 (p110) precursor contains p50 at its N-terminus and a C-terminal region that when expressed as a separate molecule, designated PDI, binds to p50 and regulates its activity.
• Synonyms: NFKB1, KBF1, EBP-1, EBP1, NF-kappa-B p50

Reference Data

• Protein Families: Druggable Genome, Transcription Factors
• Protein Pathways: Acute myeloid leukemia, Adipocytokine signaling pathway, Apoptosis, B cell receptor signaling pathway, Chemokine signaling pathway, Chronic myeloid leukemia, Cytosolic DNA-sensing pathway, Epithelial cell signaling in Helicobacter pylori infection, MAPK signaling pathway, Metabolic pathways, Neurotrophin signaling pathway, NOD-like receptor signaling pathway, Pancreatic cancer, Pathways in cancer, Prostate cancer, RIG-I-like receptor signaling pathway, Small cell lung cancer, T cell receptor signaling pathway, Toll-like receptor signaling pathway