DOK1 Rabbit Polyclonal Antibody

CAT#: AP20744PU-N

DOK1 rabbit polyclonal antibody, Aff - Purified

Product Images

Western blot (WB) analyzes of Dok-1 antibody (Cat.-No.: AP20744PU-N) in extracts from Jurkatcells.

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  Immunohistochemistry (IHC) analyzes of Dok-1 antibody (Cat.-No.: AP20744PU-N) in paraffin-embedded human breast carcinoma tissue.

Specifications

Product Data

• Applications: IF, IHC, WB
• Recommended Dilution: Western blot: 1/500 - 1/1000.
  Immunohistochemistry on paraffin sections: 1/50 - 1/200.
  Immunofluorescence: 1/50 - 1/200.
• Reactivities: Human, Mouse, Rat
• Host: Rabbit
• Clonality: Polyclonal
• Specificity: This antibody detects endogenous levels of Dok-1 protein.
  (region surrounding Pro356)
• Formulation: Phosphate buffered saline (PBS), pH 7.2
  State: Aff - Purified
  State: Liquid purified Ig fraction
  Preservative: 0.05% sodium azide
• Concentration: 1.0 mg/ml
• Purification: Affinity chromatography (> 95% (by SDS-PAGE)
• Conjugation: Unconjugated
• Storage: Store undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer.
  Avoid repeated freezing and thawing.
• Stability: Shelf life: one year from despatch.
• Predicted Protein Size: ~ 62 kDa
• Gene Name: docking protein 1
• Database Link:
  Entrez Gene 13448 MouseEntrez Gene 312477 RatEntrez Gene 1796 Human
  UniProt ID Q99704
• Background: Dok-1 associates with the Ras GTPase-activating protein (Ras GAP) upon tyrosine phosphorylation. Evidence suggests that Dok-1 (also designated p62dok) is a substrate of the constitutive tyrosine kinase activity of p210 Bcr-Abl, a fusion protein caused by the t(9;22) translocation and associated with chronic myelogenous leukemia. Dok-1, as well as the tyrosine kinase substrates IRS-1 and Cas, are members of a class of “docking” proteins which contain multiple tyrosine residues and putative SH2 binding sites. Dok-1 is suspected to be the substrate phosphorylated in response to stimulation by a number of growth factors, including PDGF, VEGF, insulin and IGF.
• Synonyms: Docking protein 1, pp62, p62(dok)

Reference Data

• Protein Families: Druggable Genome