CD19 Rabbit Polyclonal Antibody

CAT#: AP20344PU-N

CD19 rabbit polyclonal antibody, Aff - Purified

Product Images

Western blot analysis of CD19 antibody in extracts from COS7 cells.

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  Western Blot analysis of CD19 antibody at 1/500 dilution: Lane 1: MCF-7 cell lysate. Lane 2: Mouse spleen tissue lysate Lane 3: Rat spleen tissue lysate.

Specifications

Product Data

• Applications: WB
• Recommended Dilution: Western blot: 1/500-1/1000.
• Reactivities: Human, Mouse, Rat
• Host: Rabbit
• Clonality: Polyclonal
• Immunogen: Synthetic peptide corresponding to amino acids 490-540 of Human CD19.
• Specificity: This antibody detects endogenous levels of CD19 protein.
• Formulation: PBS, pH~7.2
  State: Aff - Purified
  State: Liquid purified Ig fraction
  Preservative: 0.05% Sodium Azide
• Concentration: 1.0 mg/ml
• Purification: Affinity-chromatography using epitope-specific immunogen
• Conjugation: Unconjugated
• Storage: Store undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer.
  Avoid repeated freezing and thawing.
• Stability: Shelf life: one year from despatch.
• Predicted Protein Size: ~ 61 kDa
• Gene Name: CD19 molecule
• Database Link:
  Entrez Gene 12478 MouseEntrez Gene 365367 RatEntrez Gene 930 Human
  UniProt ID P15391
• Background: CD19 is a transmembrane glycoprotein that contains two extracellular immunoglobulin- like domains. CD19 is selectively expressed on the cell surface of B lymphocytes, where it activates intracellular signaling cascades involving both Ras and phosphatidylinositol 3-kinase pathways. Activation of CD19 results in cross-linking of the membrane protein immunoglobulin chains and the subsequent association with Src family protein tyrosine kinases (PTK). Expression of CD19 is continuous throughout B cell development and through terminal differentiation of B cells into plasma cells. CD19 forms functional complexes with B lymphocyte surface proteins, including integrin β1, CD21 and CD81, which are involved in regulating B cell development.
• Synonyms: Leu-12, B-cell marker

Reference Data

• Protein Families: Druggable Genome, Transmembrane
• Protein Pathways: B cell receptor signaling pathway, Hematopoietic cell lineage, Primary immunodeficiency