GABA A Receptor gamma 2 (GABRG2) (400-410) Goat Polyclonal Antibody

CAT#: AP20117PU-N

GABA A Receptor gamma 2 (GABRG2) (400-410) goat polyclonal antibody, Aff - Purified

Product Images

GABRG2 antibody staining of Mouse Brain lysate at 2 ug/ml (RIPA buffer, 35 ug total protein per lane). Primary incubated for 1 hour. Detected by western blot using chemiluminescence.

Specifications

Product Data

• Applications: ELISA, WB
• Recommended Dilution: Peptide ELISA: Limit Dilution: 1/16000.
  Western blot: 1-3 µg/ml. Approx 50kDa band observed in Mouse and Rat Brain lysates (calculated MW of 55.1kDa according to Mouse NP_032099.1 and 54.1kDa according to Rat NP_899156.1).
• Reactivities: Bovine, Human, Mouse, Rat
• Host: Goat
• Clonality: Polyclonal
• Immunogen: Peptide with sequence from the internal region of the protein sequence according to NP_944494.1; NP_000807.2; NP_944493.2.
• Specificity: This antibody is expected to recognize all reported isoforms: NP_944494.1; NP_000807.2; NP_944493.2.
• Formulation: Tris saline, pH 7.3 containing 0.02% Sodium Azide as preservative and 0.5% BSA as stabilizer.
  State: Aff - Purified
  State: Liquid purified Ig fraction.
• Concentration: lot specific
• Purification: Ammonium Sulphate Precipitation followed by Antigen Affinity Chromatography using the immunizing peptide.
• Conjugation: Unconjugated
• Storage: Store the antibody undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer.
  Avoid repeated freezing and thawing.
• Stability: Shelf life: one year from despatch.
• Gene Name: gamma-aminobutyric acid type A receptor gamma2 subunit
• Database Link:
  Entrez Gene 14406 MouseEntrez Gene 29709 RatEntrez Gene 2566 Human
  UniProt ID P18507
• Background: GABA A receptors are ligand-gated chloride channels that play a role as inhibitory neurotransmitters. They are known targets for certain classes of environmental and pharmaceutical compounds because a number of drugs interact with binding sites on GABA A. Some of these drugs include benzodiazepines, anticonvulsants, and anesthetics.
  GABA A Receptors are comprised of subunits and these are further classified into 3 major groups (alpha, beta, and gamma). The subunits determine the pharmacological characteristics.
  The binding of GABA to GABA A receptors results in the increase transportation of chlorine ions into the neuron. This influx results in signal transmission repression. Studies have shown that if cell to cell signaling goes unchecked due to a lack of GABA, a system "overload" can resulting in blackouts, rapid body contractions, seizures and epilepsy.
• Synonyms: GABRG-2, GABA A Receptor subunit gamma-2

Reference Data

• Protein Families: Druggable Genome, Ion Channels: Cys-loop Receptors, Transmembrane
• Protein Pathways: Neuroactive ligand-receptor interaction