ALDOA Sheep Polyclonal Antibody

CAT#: AP20105PU-N

ALDOA sheep polyclonal antibody, Purified

Specifications

Product Data

• Applications: ELISA, ID, IF, IP, R, WB
• Recommended Dilution: This product is intended for use in precipitating and non-precipitating antibody-binding assays such as e.g., ELISA and Western blotting and Immunofluorescence or Histochemical techniques, to prepare an insoluble immuno-affinity adsorbent, for labelling with a marker of choice.
  Working Dilutions:
  Non-precipitating antibody-binding techniques: 1/100-1/5,000.
• Reactivities: Rabbit
• Host: Sheep
• Isotype: IgG
• Clonality: Polyclonal
• Immunogen: Aldolase isolated and purified from Rabbit muscle.
  Freund’s complete adjuvant is used in the first step of the immunization procedure.
• Specificity: The reagents were evaluated for potency, purity and specificity using most or all of the following techniques: Immunoelectrophoresis, Cross-Immunoelectrophoresis, single Radial Immunodiffusion (Ouchterlony), block titration, ELISA, Immunoblotting and Enzyme Inhibition.
  Cross-reactivities against enzymes of other sources may occur but have not been determined.
• Formulation: PBS, pH 7.2 stabilized with Dextran without preservatives and foreign proteins
  State: Purified
  State: Lyophilized purified IgG fraction
• Reconstitution Method: Restore by adding 0.5 ml of sterile distilled water
• Concentration: lot specific
• Purification: Solid Phase Affinity Chromatography
• Conjugation: Unconjugated
• Storage: Store the antibody lyophilized at 2-8°C and reconstituted at 2-8°C for one week or (in aliquots) at -20°C for longer.
  If a slight precipitation occurs upon storage, this should be removed by centrifugation.
• Stability: Shelf life: one year from despatch.
• Database Link:
  Entrez Gene 100009055 Rabbit
  UniProt ID P00883
• Background: This gene product, Aldolase A (fructose bisphosphate aldolase) is a glycolytic enzyme that catalyzes the reversible conversion of fructose-1,6-bisphosphate to glyceraldehyde 3 phosphate and dihydroxyacetone phosphate. Three aldolase isozymes (A, B, and C), encoded by three different genes, are differentially expressed during development. Aldolase A is found in the developing embryo and is produced in even greater amounts in adult muscle. Aldolase A expression is repressed in adult liver, kidney and intestine and similar to aldolase C levels in brain and other nervous tissue. Aldolase A deficiency has been associated with myopathy and hemolytic anemia. Alternative splicing of this gene results in multiple transcript variants which encode the same protein.
• Synonyms: ALDA, NY-LU-1, Muscle-type aldolase