LTA (Center) Rabbit Polyclonal Antibody

CAT#: AP17540PU-N

LTA (Center) rabbit polyclonal antibody

Product Images

Western blot analysis of LTA Antibody (Center) in MDA-MB231 cell line lysates (35ug/lane). LTA (arrow) was detected using the purified Pab.

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  Formalin-fixed and paraffin-embedded human hepatocarcinoma reacted with LTA Antibody (Center), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining.

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  Flow Cytometric analysis of HepG2 cells using LTA Antibody (Center) (bottom histogram) compared to a negative control cell (top histogram). FITC-conjugated Goat-anti-Rabbit secondary antibodies were used for the analysis.

Specifications

Product Data

• Applications: FC, IHC, WB
• Recommended Dilution: ELISA: 1/1000.
  Western blotting: ~1/1000.
  Flow Cytometry: 1/10-1/50.
  Immunohistochemistry on Paraffin Sections: 1/50-1/100.
• Reactivities: Human
• Host: Rabbit
• Clonality: Polyclonal
• Immunogen: KLH conjugated synthetic peptide between 46-72 amino acids from the Central region of Human LTA.
• Specificity: This antibody recognizes Human TNF-beta / Lymphotoxin-alpha.
  Other species not tested.
• Formulation: PBS
  State: Liquid purified Ig fraction
  Preservative: 0.09% (W/V) Sodium Azide
• Concentration: lot specific
• Purification: Peptide Affinity Chromatography on Protein A
• Conjugation: Unconjugated
• Storage: Store undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer.
  Avoid repeated freezing and thawing.
• Stability: Shelf life: one year from despatch.
• Predicted Protein Size: 22297 Da
• Gene Name: lymphotoxin alpha
• Database Link:
  Entrez Gene 4049 Human
  UniProt ID P01374
• Background: LTA, a member of the tumor necrosis factor family, is a cytokine produced by lymphocytes. The protein is highly inducible, secreted, and forms heterotrimers with lymphotoxin-beta which anchor lymphotoxin-alpha to the cell surface. This protein also mediates a large variety of inflammatory, immunostimulatory, and antiviral responses, is involved in the formation of secondary lymphoid organs during development and plays a role in apoptosis.
• Synonyms: TNFB, TNFSF1, LTA, LT-alpha

Reference Data

• Protein Families: Druggable Genome, Secreted Protein, Transmembrane
• Protein Pathways: Antigen processing and presentation, Cytokine-cytokine receptor interaction, Type I diabetes mellitus