BLNK (Center) Rabbit Polyclonal Antibody

CAT#: AP17153PU-N

BLNK (Center) rabbit polyclonal antibody

Product Images

Western blot analysis of BLNK Antibody (Center) in NCI-H460 cell line lysates (35ug/lane). BLNK (arrow) was detected using the purified Pab.

Specifications

Product Data

• Applications: WB
• Recommended Dilution: ELISA:1/1,000.
  Western Blot: 1/100~1/500.
• Reactivities: Human
• Host: Rabbit
• Clonality: Polyclonal
• Immunogen: This BLNK antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide selected from the Center region of human BLNK.
• Specificity: BLNK antibody recognizes BLNK at Center.
• Formulation: PBS with 0.09% (W/V) Sodium Azide as preservative
  State: Liquid purified Ig fraction.
• Concentration: lot specific
• Purification: Protein A column followed by Peptide Affinity Purification.
• Conjugation: Unconjugated
• Storage: Store the antibody undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer.
  Avoid repeated freezing and thawing.
• Stability: Shelf life: one year from despatch.
• Gene Name: B-cell linker
• Database Link:
  Entrez Gene 29760 Human
  UniProt ID Q8WV28
• Background: BLNK (B cell linker protein) is an adaptor molecule that plays key role in B cell activation initiated by cross-linking the B cell receptor (BCR). This activation leads to an increase in the tyrosine phosphorylation of a number of proteins due to activation of nonreceptor protein tyrosine kinases (PTK) including Syk, which is responsible for the tyrosine phosphorylation of BLNK. BLNK is cytosolic protein identified as two phosphoproteins migrating at 68 and 70 kDa in SDS/PAGE (alternatively spliced forms of human BLNK). Mapping of the tyrosine residues in BLNK reveal three tyrosines that bind PLC-gamma2, and two tyrosines that bind the Tec family kinase Btk, the Vav nucleotide exchange factor and the adaptor Nck.
• Synonyms: BASH, SLP65, SLP-65

Reference Data

• Protein Families: Druggable Genome
• Protein Pathways: B cell receptor signaling pathway, Primary immunodeficiency