B Raf (BRAF) Rabbit Polyclonal Antibody

CAT#: AP14616PU-N

B Raf (BRAF) rabbit polyclonal antibody, Aff - Purified

Product Images

Western blot analysis of BRAF (arrow) using rabbit polyclonal BRAF Antibody (S445). 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the BRAF gene (Lane 2)

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  Formalin-fixed and paraffin-embedded human brain tissue reacted with BRAF Antibody (S445), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining.

Specifications

Product Data

• Applications: IHC, WB
• Recommended Dilution: Peptide ELISA: 1/1,000.
  Western blotting: 1/1,000.
  Immunohistochemistry: 1/10-1/50.
• Reactivities: Human
• Host: Rabbit
• Isotype: Ig
• Clonality: Polyclonal
• Immunogen: KLH conjugated synthetic peptide corresponding to amino acid between 425-453 from human BRAF.
• Specificity: This antibody reacts to B-RAF around Ser445.
• Formulation: PBS with 0.09% (W/V) sodium azide
  State: Aff - Purified
  State: Liquid purified Ig
• Concentration: lot specific
• Purification: Protein A column, followed by peptide affinity purification
• Conjugation: Unconjugated
• Storage: Store the antibody undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer.
  Avoid repeated freezing and thawing.
• Stability: Shelf life: one year from despatch.
• Gene Name: B-Raf proto-oncogene, serine/threonine kinase
• Database Link:
  Entrez Gene 673 Human
  UniProt ID P15056
• Background: BRAF, a member of the RAF subfamily of Ser/Thr protein kinases, is involved in the transduction of mitogenic signals from the cell membrane to the nucleus. It may play a role in the postsynaptic responses of hippocampal neurons. This cytoplasmic protein is expressed in brain and testis. Defects in BRAF are involved in a wide range of cancers including lung cancer and non-Hodgkin lymphoma (NHL). This protein contains 1 zinc-dependent phorbol-ester and DAG binding domain.
• Synonyms: BRAF, BRAF1, RAFB1, p94