MCK10 (DDR1) Rabbit Polyclonal Antibody

CAT#: AP14383PU-N

MCK10 (DDR1) rabbit polyclonal antibody, Purified

Product Images

Western blot analysis of anti-MCK10 Pab in SK-BR-3 cell line lysates (35ug/lane).MCK10 (arrow) was detected using the purified Pab (1:60 dilution).

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  Formalin-fixed and paraffin-embedded human lung carcinoma tissue reacted with MCK10 antibody, which was peroxidase-conjugated to the secondary antibody, followed by DAB staining.

Specifications

Product Data

• Applications: IHC, WB
• Recommended Dilution: ELISA: 1/1,000.
  Western blotting: 1/50 - 1/100.
  Immunohistochemistry: 1/10 - 1/50.
• Reactivities: Human
• Host: Rabbit
• Isotype: Ig
• Clonality: Polyclonal
• Immunogen: This antibody is generated from rabbits immunized with recombinant human MCK10 protein.
• Specificity: This antibody reacts to MCK10.
• Formulation: PBS with 0.09% (W/V) sodium azide
  State: Purified
  State: Liquid purified Ig
• Concentration: lot specific
• Purification: Prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS
• Conjugation: Unconjugated
• Storage: Store the antibody undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer.
  Avoid repeated freezing and thawing.
• Stability: Shelf life: one year from despatch.
• Gene Name: discoidin domain receptor tyrosine kinase 1
• Database Link:
  Entrez Gene 780 Human
  UniProt ID Q08345
• Background: Receptor tyrosine kinases (RTKs) play a key role in the communication of cells with their microenvironment. These molecules are involved in the regulation of cell growth, differentiation and metabolism. MCK10 is a RTK that is widely expressed in normal and transformed epithelial cells and is activated by various types of collagen. This protein belongs to a subfamily of tyrosine kinase receptors with a homology region to the Dictyostelium discoideum protein discoidin I in their extracellular domain. Its autophosphorylation is achieved by all collagens so far tested (type I to type VI). In situ studies and Northern-blot analysis showed that expression of this encoded protein is restricted to epithelial cells, particularly in the kidney, lung, gastrointestinal tract, and brain. In addition, this protein is significantly over-expressed in several human tumors from breast, ovarian, esophageal, and pediatric brain.
• Synonyms: Tyrosine kinase DDR-1, CAK, EDDR1, NEP, NTRK4, PTK3A, RTK6, TRKE, MCK10, Epithelial discoidin domain receptor 1