MAPK15 (N-term) Rabbit Polyclonal Antibody

CAT#: AP14193PU-N

MAPK15 (N-term) rabbit polyclonal antibody, Purified

Product Images

(LEFT) Western blot analysis of ERK8 (arrow) using rabbit polyclonal ERK8 Antibody (N-term). 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the Mrified Pab (1:60 dilution).

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  Formalin-fixed and paraffin-embedded human breast carcinoma tissue reacted with ERK8 antibody (N-term), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining.

Specifications

Product Data

• Applications: IHC, WB
• Recommended Dilution: ELISA: 1/1,000.
  Western blotting: 1/50 - 1/100.
  Immunohistochemistry: 1/10 - 1/50.
• Reactivities: Human
• Host: Rabbit
• Isotype: Ig
• Clonality: Polyclonal
• Immunogen: This antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide selected from the N-terminal region of human ERK8.
• Specificity: This antibody reacts to MAPK15 (ERK8).
• Formulation: PBS with 0.09% (W/V) sodium azide
  State: Purified
  State: Liquid purified Ig
• Concentration: lot specific
• Purification: Protein A column, eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS
• Conjugation: Unconjugated
• Storage: Store the antibody undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer.
  Avoid repeated freezing and thawing.
• Stability: Shelf life: one year from despatch.
• Gene Name: mitogen-activated protein kinase 15
• Database Link:
  Entrez Gene 225689 Human
  UniProt ID Q8TD08
• Background: The ERKs are a subfamily of the MAPKs that have been implicated in cell growth and differentiation. Extracellular signal-regulated kinase 8 (Erk8) is a large MAP kinase whose activity is controlled by serum and the c-Src non-receptor tyrosine kinase. ERK8 down-regulates transactivation of the glucocorticoid receptor through Hic-5 and can negatively regulate transcriptional co-activation of androgen receptor and GRalpha by Hic-5 in a kinase-independent manner, suggesting a broader role for ERK8 in the regulation of nuclear receptors beyond estrogen receptor alpha. Erk8 is a novel effector of RET/PTC3 and, therefore, RET biological functions.
• Synonyms: Mitogen-activated protein kinase 15, ERK8