AMPK alpha 1 (PRKAA1) Rabbit Polyclonal Antibody

CAT#: AP13965PU-N

AMPK alpha 1 (PRKAA1) rabbit polyclonal antibody, Aff - Purified

Product Images

Western blot analysis of PRKAA1 (arrow) using rabbit polyclonal PRKAA1 Antibody. 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the PRKAA1 gene (Lane 2) (Origene Technologies).

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  Formalin-fixed and paraffin-embedded human breast carcinoma tissue reacted with PRKAA1, which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.

Specifications

Product Data

• Applications: IHC, WB
• Recommended Dilution: ELISA 1:1,000.
  Western blot 1:50 - 1:100.
  Immunohistochemistry 1:10 - 1:50.
• Reactivities: Human
• Host: Rabbit
• Isotype: Ig
• Clonality: Polyclonal
• Immunogen: This antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide corresponding to amino acid residues surrounding S487 of human PRKAA1.
• Specificity: This antibody detects AMPK alpha (PRKAA1).
• Formulation: PBS with 0.09% (W/V) sodium azide
  State: Aff - Purified
  State: Liquid Ig fraction
• Concentration: lot specific
• Purification: Protein A column, followed by peptide affinity purification.
• Conjugation: Unconjugated
• Storage: Store the antibody at 2 - 8 °C up to one month or (in aliquots) at -20 °C for longer. Avoid repeated freezing and thawing.
• Stability: Shelf life: one year from despatch.
• Gene Name: protein kinase AMP-activated catalytic subunit alpha 1
• Database Link:
  Entrez Gene 5562 Human
  UniProt ID Q13131
• Background: PRKAA1 belongs to the ser/thr protein kinase family. It is the catalytic subunit of the 5'-prime-AMP-activated protein kinase (AMPK). AMPK is a cellular energy sensor conserved in all eukaryotic cells. The kinase activity of AMPK is activated by the stimuli that increase the cellular AMP/ATP ratio. AMPK regulates the activities of a number of key metabolic enzymes through phosphorylation. It protects cells from stresses that cause ATP depletion by switching off ATP-consuming biosynthetic pathways.
• Synonyms: AMPK1, AMPK alpha-1 chain
• Note: Molecular weight: 63878 Da