Metabotropic Glutamate Receptor 5 (GRM5) (C-term) Rabbit Polyclonal Antibody

CAT#: AP13414PU-N

Metabotropic Glutamate Receptor 5 (GRM5) (C-term) rabbit polyclonal antibody, Purified

Product Images

Western blot analysis of Metabotropic Glutamate Receptor 5 (GPRC1E) antibody (C-term) in mouse brain tissue lysates (35ug/lane). Metabotropic Glutamate Receptor 5 (arrow) was detected using the purified Pab.

Specifications

Product Data

• Applications: WB
• Recommended Dilution: ELISA: 1/1,000.
  Western Blot.
• Reactivities: Human
• Host: Rabbit
• Isotype: Ig
• Clonality: Polyclonal
• Immunogen: This antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide selected from the C-terminal region of human GPRC1E.
• Specificity: This antibody reacts to Metabotropic Glutamate Receptor 5 (GPRC1E).
• Formulation: PBS with 0.09% (W/V) sodium azide
  State: Purified
  State: Liquid purified Ig
• Concentration: lot specific
• Purification: Protein G column, eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS
• Conjugation: Unconjugated
• Storage: Store the antibody undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer.
  Avoid repeated freezing and thawing.
• Stability: Shelf life: one year from despatch.
• Gene Name: glutamate metabotropic receptor 5
• Database Link:
  Entrez Gene 2915 Human
  UniProt ID P41594
• Background: L-glutamate is the major excitatory neurotransmitter in the central nervous system and activates both ionotropic and metabotropic glutamate receptors. Glutamatergic neurotransmission is involved in most aspects of normal brain function and can be perturbed in many neuropathologic conditions. The metabotropic glutamate receptors are a family of G protein-coupled receptors, that have been divided into 3 groups on the basis of sequence homology, putative signal transduction mechanisms, and pharmacologic properties. Group I includes GRM1 and GRM5 (also known as GPRC1E) and these receptors have been shown to activate phospholipase C. Group II includes GRM2 and GRM3 while Group III includes GRM4, GRM6, GRM7 and GRM8. Group II and III receptors are linked to the inhibition of the cyclic AMP cascade but differ in their agonist selectivities. The activity of GRM5 is mediated by a G-protein that activates a phosphatidylinositol-calcium second messenger system and generates a calcium-activated chloride current.
• Synonyms: Metabotropic glutamate receptor 5, GPRC1E, MGLUR5