Glypican 3 (GPC3) (Center) Rabbit Polyclonal Antibody

CAT#: AP13408PU-N

Glypican 3 (GPC3) (Center) rabbit polyclonal antibody, Purified

Product Images

Western blot analysis in Mouse stomach tissue lysates using GPC3 Antibody (35ug/lane). This demonstrates the GPC3 antibody detected the GPC3 protein (arrow).

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  Western blot analysis in HepG2 cell line lysates using GPC3 Antibody (35ug/lane). This demonstrates the GPC3 antibody detected the GPC3 protein (arrow).

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  Immunofluorecence staining of GPC3 Antibody on HepG2 cells. The cells were acetone fixated. Antibody dilution of 1/50. Original magnification 1/400. Data and protocol courtesy of Dr. Mariana Dabeva, Department of Medicine at Albert Einstein College of Medicine.

Specifications

Product Data

• Applications: IF, WB
• Recommended Dilution: ELISA: 1/1,000.
  Western blot: 1/50-1/100.
  Immunofluorescence.
• Reactivities: Human
• Host: Rabbit
• Isotype: Ig
• Clonality: Polyclonal
• Immunogen: KLH conjugated synthetic peptide selected from the center region of Human GPC3 (aa 345-370)
• Specificity: This antibody recognizes Human and Mouse Glypican 3 (GPC3).
  Other species not tested.
• Formulation: PBS containing 0.09% (W/V) Sodium Azide as preservative
  State: Purified
  State: Liquid purified Ig fraction
• Concentration: lot specific
• Purification: Protein G Column, eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS
• Conjugation: Unconjugated
• Storage: Store undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer.
  Avoid repeated freezing and thawing.
• Stability: Shelf life: one year from despatch.
• Gene Name: glypican 3
• Database Link:
  Entrez Gene 2719 Human
  UniProt ID P51654
• Background: GPC3 is a cell surface proteoglycan that bears heparan sulfate. This protein may be involved in the suppression/modulation of growth in the predominantly mesodermal tissues and organs, and may play a role in the modulation of IGF2 interactions with its receptor and thereby modulate its function. Members of the glypican-related integral membrane proteoglycan family contain a core protein anchored to the cytoplasmic membrane via a glycosyl phosphatidylinositol (GPI) linkage. These proteins may play a role in the control of cell division, growth regulation, and tumor predisposition. Deletion mutations in GPC3 are the cause of Simpson-Golabi-Behmel syndrome (SGBS), also known as Simpson dysmorphia syndrome (SDYS). SGBS is a condition characterized by pre- and postnatal overgrowth (gigantism) with visceral and skeletal anomalies.
• Synonyms: Intestinal protein OCI-5, GTR2-2, MXR7, OCI5