SMAD4 pThr277 Rabbit Polyclonal Antibody

CAT#: AP12711PU-N

SMAD4 pThr277 rabbit polyclonal antibody, Aff - Purified

Product Images

Formalin-fixed and paraffin-embedded Breast Carcinoma reacted with SMAD4 pThr277 (center), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.

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  Dot blot analysis of anti-Phospho-SMAD4 pThr277 Antibody on nitrocellulose membrane. 50ng of Phospho-peptide or Non Phospho-peptide per dot were adsorbed. Antibody working concentrations are 0.5ug per ml.

Specifications

Product Data

• Applications: IHC
• Recommended Dilution: ELISA: 1/1,000.
  Dot Blot: 1/500.
  Immunohistochemistry: 1/50-1/100.
• Reactivities: Human
• Host: Rabbit
• Isotype: Ig
• Clonality: Polyclonal
• Immunogen: This antibody is generated from rabbits immunized with a KLH-conjugated synthetic phosphopeptide corresponding to amino acid residues surrounding T277 of human SMAD4.
• Specificity: This antibody detects SMAD4 pThr277.
• Formulation: PBS with 0.09% (W/V) Sodium Azide as preservative.
  State: Aff - Purified
  State: Liquid purified Ig fraction.
• Concentration: lot specific
• Purification: Protein G Chromatography, eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS.
• Conjugation: Unconjugated
• Storage: Store the antibody undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer.
  Avoid repeated freezing and thawing.
• Stability: Shelf life: one year from despatch.
• Gene Name: SMAD family member 4
• Database Link:
  Entrez Gene 4089 Human
  UniProt ID Q13485
• Background: Common mediator of signal transduction by TGF-beta (transforming growth factor) superfamily, SMAD4 is the common SMAD (co-SMAD). It promotes binding of the SMAD2/SMAD4/FAST-1 complex to DNA and provides an activation function required for SMAD1 or SMAD2 to stimulate transcription. It may act as a tumor suppressor.
• Synonyms: SMAD family member 4, SMAD-4, SMAD 4, MADH4, MAD homolog 4, DPC4
• Note: Molecular Weight: 60439 Da