IKK beta (IKBKB) pTyr199 Rabbit Polyclonal Antibody

CAT#: AP12624PU-N

IKK beta (IKBKB) pTyr199 rabbit polyclonal antibody, Aff - Purified

Product Images

The anti-Phospho-IKKb-Y199 Pab is used in Western blot to detect Phospho-IKKb-Y199 in Y79 tissue lysate

Specifications

Product Data

• Applications: WB
• Recommended Dilution: ELISA: 1/1,000.
  Western Blot: 1/100-1/500.
• Reactivities: Human
• Host: Rabbit
• Isotype: Ig
• Clonality: Polyclonal
• Immunogen: This antibody is generated from rabbits immunized with a KLH conjugated synthetic phosphopeptide corresponding to amino acid residues surrounding Y199 of human IKKb.
• Specificity: This antibody detects IKKb pTyr199.
  Predicted to cross react with Mouse (100% Antigen Homology).
• Formulation: PBS with 0.09% (W/V) Sodium Azide as preservative.
  State: Aff - Purified
  State: Liquid purified Ig fraction.
• Concentration: lot specific
• Purification: Protein G Affinity Chromatography. Then, the antibody fraction is peptide affinity purified in a 2-step procedure with control and phosphorylated peptides. The phospho-specific antibody is eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS.
• Conjugation: Unconjugated
• Storage: Store the antibody undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer.
  Avoid repeated freezing and thawing.
• Stability: Shelf life: one year from despatch.
• Gene Name: inhibitor of kappa light polypeptide gene enhancer in B-cells, kinase beta
• Database Link:
  Entrez Gene 3551 Human
  UniProt ID O14920
• Background: NFKB1 or NFKB2 is bound to REL, RELA, or RELB to form the NFKB complex. The NFKB complex is inhibited by I-kappa-B proteins (NFKBIA or NFKBIB), which inactivate NF-kappa-B by trapping it in the cytoplasm. Phosphorylation of serine residues on the inhibitory I-kappa-B proteins by kinases (IKKa or IKKb) leads to the dissociation of the inhibitor/NF-kappa-B complex and marks the inhibitors for destruction via the ubiquitination pathway, thereby allowing activation of the NF-kappa-B complex. Activated NFKB complex translocates into the nucleus and binds DNA at kappa-B-binding motifs.
• Synonyms: I-kappa-B-kinase beta, IKK-beta, IKK-B, I-kappa-B kinase 2, IKK2, NFKBIKB
• Note: Molecular weight: 86564 Da