CREB1 pSer133 Rabbit Polyclonal Antibody

CAT#: AP12596PU-N

CREB1 pSer133 rabbit polyclonal antibody, Aff - Purified

Product Images

Western blot analysis of anti-Phospho-CREB pSer133 Pab in CEM cell line tissue lysate (35ug/lane).Phospho-CREB-pS133 (arrow) was detected using the purified Pab (1:40 dilution).

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  Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by AEC staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.

Specifications

Product Data

• Applications: IHC, WB
• Recommended Dilution: ELISA: 1/1,000.
  Western Blot: 1/100-1/500.
  Immunohistochemistry: 1/50-1/100.
• Reactivities: Human
• Host: Rabbit
• Isotype: Ig
• Clonality: Polyclonal
• Immunogen: This antibody is generated from rabbits immunized with a KLH conjugated synthetic phosphopeptide corresponding to amino acid residues surrounding S133 of human CREB.
• Specificity: This antibody detects CREB pSer133.
  Predicted to cross react with Mouse (100% Antigen Homology).
• Formulation: PBS with 0.09% (W/V) Sodium Azide as preservative.
  State: Aff - Purified
  State: Liquid purified Ig fraction.
• Concentration: lot specific
• Purification: Protein G Affinity Chromatography. Then, the antibody fraction is peptide affinity purified in a 2-step procedure with control and phosphorylated peptides. The phospho-specific antibody is eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS.
• Conjugation: Unconjugated
• Storage: Store the antibody undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer.
  Avoid repeated freezing and thawing.
• Stability: Shelf life: one year from despatch.
• Gene Name: cAMP responsive element binding protein 1
• Database Link:
  Entrez Gene 1385 Human
  UniProt ID P16220
• Background: CREB is a transcription factor that is a member of the leucine zipper family of DNA binding proteins. This protein binds as a homodimer to the cAMP-responsive element, (CRE), a sequence present in many viral and cellular promoters. The protein is phosphorylated by several protein kinases, and induces transcription of genes in response to hormonal stimulation of the cAMP pathway.
• Synonyms: CREB-1
• Note: Molecular weight: 36688 Da