CDC25A pSer279 Rabbit Polyclonal Antibody

CAT#: AP12578PU-N

CDC25A pSer279 rabbit polyclonal antibody, Aff - Purified

Product Images

The anti-Phospho-CDC25A-S278 Pab (Cat.#AP12578PU-N) is used in Western blot to detect Phospho-CDC25A-S278 in mouse heart tissue lysate

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  Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by AEC staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.

Specifications

Product Data

• Applications: IHC, WB
• Recommended Dilution: ELISA: 1/1,000.
  Western Blot: 1/100-1/500.
  Immunohistochemistry: 1/50-1/100.
• Reactivities: Human
• Host: Rabbit
• Isotype: Ig
• Clonality: Polyclonal
• Immunogen: This antibody is generated from rabbits immunized with a KLH conjugated synthetic phosphopeptide corresponding to amino acid residues surrounding S278 of human Cdc25A.
• Specificity: This antibody detects CDC25A pSer278.
• Formulation: State: Aff - Purified
• Concentration: lot specific
• Conjugation: Unconjugated
• Storage: Store the antibody undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer.
  Avoid repeated freezing and thawing.
• Stability: Shelf life: one year from despatch.
• Gene Name: cell division cycle 25A
• Database Link:
  Entrez Gene 993 Human
  UniProt ID P30304
• Background: CDC25A is a member of the CDC25 family of phosphatases. CDC25A is required for progression from G1 to the S phase of the cell cycle. It activates the cyclin-dependent kinase CDC2 by removing two phosphate groups. CDC25A is specifically degraded in response to DNA damage, which prevents cells with chromosomal abnormalities from progressing through cell division. CDC25A is an oncogene, although its exact role in oncogenesis has not been demonstrated. Two transcript variants encoding different isoforms have been found for this gene.
• Synonyms: CDC-25A
• Note: Molecular weight: 59087 Da