UBA1 (C-term) Rabbit Polyclonal Antibody

CAT#: AP11969PU-N

UBA1 (C-term) rabbit polyclonal antibody, Purified

Product Images

The anti-UBE1 Pab is used in Western blot to detect UBE1 in HL-60 cell lysate.

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  (LEFT) Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by AEC staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. (RIGHT) Formalin-fixed and paraffin-embedded human hepatocarcinoma tissue reacted with UBE1 antibody (C-term), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.

Specifications

Product Data

• Applications: IHC, WB
• Recommended Dilution: ELISA: 1/1,000.
  Western Blot: 1/100-1/500.
  Immunohistochemistry: 1/50-1/100.
• Reactivities: Human
• Host: Rabbit
• Isotype: Ig
• Clonality: Polyclonal
• Immunogen: This antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide selected from the C-terminal region of human UBE1.
• Specificity: This antibody is specific to UBE1/UBA1 (C-term).
  Predicted to cross react with Mouse and Rat (100% Antigen Homology).
• Formulation: PBS with 0.09% (W/V) Sodium Azide as preservative.
  State: Purified
  State: Liquid purified Ig fraction.
• Concentration: lot specific
• Purification: Protein G Chromatography, eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS.
• Conjugation: Unconjugated
• Storage: Store the antibody undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer.
  Avoid repeated freezing and thawing.
• Stability: Shelf life: one year from despatch.
• Gene Name: ubiquitin like modifier activating enzyme 1
• Database Link:
  Entrez Gene 7317 Human
  UniProt ID P22314
• Background: UBE1 catalyzes the first step in ubiquitin conjugation to mark cellular proteins for degradation. This gene complements an X-linked mouse temperature-sensitive defect in DNA synthesis, and thus may function in DNA repair. It is part of a gene cluster on chromosome Xp11.23. Alternative splicing results in 2 transcript variants encoding the same protein, but with different 5' UTR.
• Synonyms: Ubiquitin-activating enzyme E1, Protein A1S9, A1S9T
• Note: Predicted Molecular weight: 117848 Da