FAU Rabbit Polyclonal Antibody

CAT#: AP11620PU-N

FAU rabbit polyclonal antibody, Purified

Product Images

Western blot analysis of anti-FUBI Center in Hela and T47D cell line lysates. FUBI Center (arrow) was detected using the purified Pab.

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  Formalin-fixed and paraffin-embedded human colon carcinoma reacted with FUBI Antibody (Center), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.

Specifications

Product Data

• Applications: IHC, WB
• Recommended Dilution: ELISA 1:1,000.
  Western blot 1:250 - 1:500.
  Immunohistochemistry 1:50 - 1:100.
• Reactivities: Human, Mouse
• Host: Rabbit
• Isotype: Ig
• Clonality: Polyclonal
• Immunogen: This antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide selected from the center region of human FUBI.
• Specificity: This antibody detects FUBI at Center.
• Formulation: PBS with 0.09% (W/V) sodium azide
  State: Purified
  State: Liquid Ig fraction
• Concentration: lot specific
• Purification: Protein G column, eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS
• Conjugation: Unconjugated
• Storage: Store the antibody at 2 - 8 °C up to one month or (in aliquots) at -20 °C for longer. Avoid repeated freezing and thawing.
• Stability: Shelf life: one year from despatch.
• Gene Name: Finkel-Biskis-Reilly murine sarcoma virus (FBR-MuSV) ubiquitously expressed
• Database Link:
  Entrez Gene 2197 Human
  UniProt ID P35544
• Background: This gene is the cellular homolog of the fox sequence in the Finkel-Biskis-Reilly murine sarcoma virus (FBR-MuSV). It encodes a fusion protein consisting of the ubiquitin-like protein fubi at the N terminus and ribosomal protein S30 at the C terminus. It has been proposed that the fusion protein is post-translationally processed to generate free fubi and free ribosomal protein S30. Fubi is a member of the ubiquitin family, and ribosomal protein S30 belongs to the S30E family of ribosomal proteins. Whereas the function of fubi is currently unknown, ribosomal protein S30 is a component of the 40S subunit of the cytoplasmic ribosome. Pseudogenes derived from this gene are present in the genome. Similar to ribosomal protein S30, ribosomal proteins S27a and L40 are synthesized as fusion proteins with ubiquitin.
• Synonyms: asr1; FAU1; FLJ22986; Fub1; Fubi; MNSFbeta; RPS30
• Note: Molecular weight: 14630 Da