ACTC1 Rabbit Polyclonal Antibody

CAT#: AP11506PU-N

ACTC1 rabbit polyclonal antibody, Purified

Product Images

Western blot analysis of ACTB/ACTC Antibody (Center) in HL60 cell line lysates (35 ug/lane). Target protein (arrow) was detected using the purified Pab (1:60 dilution).

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  Western blot analysis of ACTB/ACTC (arrow) using rabbit polyclonal ACTB/ACTC Antibody (Center). 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the ACTB/ACTC gene (Lane 2) (Origene Technologies).

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  Formalin-fixed and paraffin-embedded human skeletal muscle tissue reacted with ACTB/ACTC antibody (Center), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.

Specifications

Product Data

• Applications: IHC, WB
• Recommended Dilution: ELISA 1:1,000.
  Western blot 1:50 - 1:100.
  Immunohistochemistry 1:10 - 1:50.
• Reactivities: Human
• Host: Rabbit
• Isotype: Ig
• Clonality: Polyclonal
• Immunogen: This antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide selected from the center region of human ACTB/ACTC.
• Specificity: This antibody detects Actin (ACTB/ACTC) at center.
• Formulation: PBS with 0.09% (W/V) sodium azide
  State: Purified
  State: Liquid Ig fraction
• Concentration: lot specific
• Purification: Prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.
• Conjugation: Unconjugated
• Storage: Store the antibody at 2 - 8 °C up to one month or (in aliquots) at -20 °C for longer. Avoid repeated freezing and thawing.
• Stability: Shelf life: one year from despatch.
• Gene Name: actin, alpha, cardiac muscle 1
• Database Link:
  Entrez Gene 70 Human
  UniProt ID P68032
• Background: Actins are highly conserved proteins that are involved in cell motility, structure, and integrity. ACTB/ACTC are nonmuscle cytoskeletal actins and major constituents of the contractile apparatus. Defects in ACTB are a cause of juvenile-onset dystonia. Defects in ACTC have been associated with idiopathic dilated cardiomyopathy (IDC) and familial hypertrophic cardiomyopathy (FHC).
• Synonyms: ACTC; ASD5; CMD1R; CMH11
• Note: Molecular weight: 41737 Da / 42019 Da