Biotin (F(ab')2 fragment) Goat Polyclonal Antibody

CAT#: AP09429TC-N

Biotin (F(ab')2 fragment) goat polyclonal antibody, TRITC

Specifications

Product Data

• Applications: FC, IF
• Recommended Dilution: Immunofluorescence.
  Flow Cytometry.
• Host: Goat
• Isotype: IgG
• Clonality: Polyclonal
• Immunogen: Biotin conjugated to Keyhole Limpet Hemocyanin (KLH)
• Specificity: This is a Rhodamine Conjugated F(ab)2 fragment of Anti-Biotin.
• Formulation: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2 containing 10 mg/ml Bovine Serum Albumin (BSA) and 0.01% (w/v) Sodium Azide
  Label: TRITC
  State: Lyophilized F(ab)2 fragment
  Label: Tetramethylrhodamine isothiocyanate (Molecular Weight 444 daltons)
  Absorption emission: 550 nm / 570 nm
  Molar radio: 4.0 moles TRITC per mole of Goat IgG F(ab’)2
• Reconstitution Method: Restore with 1.0 ml of deionized water (or equivalent).
• Concentration: lot specific
• Purification: Immunoaffinity chromatography
• Conjugation: TRITC
• Storage: Prior to reconstitution store at 2-8°C.
  Following reconstitution store the antibody undiluted at 2-8°C for one month
  or (in aliquots) at -20°C for longer.
  Avoid repeated freezing and thawing.
• Stability: Shelf life: one year from despatch.
• Background: Biotin is a water soluble vitamin, generally classified as a B complex vitamin, also called vitamin B4. After the initial discovery of biotin, nearly forty years of research were required to establish it as a vitamin. Biotin is required by all organisms but can only be synthesized by bacteria, yeasts, molds, algae, and some plant species. Biotin is required as prosthetic group of enzymes involved in incorporation of carbon dioxide into organic compounds. Biotin has a MW of 244 Da.
• Synonyms: Vitamin B7, Vitamin H