Protein G Rabbit Polyclonal Antibody

CAT#: AP09167TR-N

Protein G rabbit polyclonal antibody, Texas Red

Specifications

Product Data

• Applications: ELISA, FC
• Recommended Dilution: Immunofluorescence.
  Flow Cytometry.
• Reactivities: Streptococcus
• Host: Rabbit
• Isotype: IgG
• Clonality: Polyclonal
• Immunogen: Protein G [Streptococcus species]
• Specificity: This antibody reacts to Protein G.
• Formulation: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2 containing 10 mg/ml Bovine Serum Albumin (BSA) as stabilizer and 0.01% (w/v) Sodium Azide as preservative
  Label: Texas Red
  State: Lyophilized IgG fraction
  Label: (TM) Sulfonyl Chloride (Molecular Weight 625 dalton
  Absorption emission: 596 nm / 620 nm
  Molar radio: 2.4 moles Texas Red(TM) per mole of Rabbit IgG
• Reconstitution Method: Restore with 2.0 ml of deionized water (or equivalent).
  For extended storage reconstitute product with 50% glycerol.
• Concentration: lot specific
• Purification: Ion exchange chromatography
• Conjugation: Texas Red
• Storage: Prior to reconstitution store at 2-8°C.
  Following reconstitution store the antibody undiluted at 2-8°C for one month
  or (in aliquots) at -20°C for longer.
  Avoid repeated freezing and thawing.
• Stability: Shelf life: one year from despatch.
• Background: Protein G is a bacterial protein derived from the cell wall of certain strains of b-hemolytic Streptococcci. It binds with high affinity to the Fc portion of various classes and subclasses of immunoglobulins from a variety of species. Protein G binds to all IgG subclasses from human, mouse and rat species. It also binds to total IgG from guinea pig, rabbit, goat, cow, sheep, and horse.
  Protein G binds preferentially to the Fc portion of IgG, but unlike Protein A can also bind to the Fab region, making it useful for purification of F(ab') fragments of IgG. Due to it's affinity for the Fc region of many mammalian immunoglobulins, protein G is considered a universal reagent in biochemistry and immunology.