Protein A Goat Polyclonal Antibody

CAT#: AP09121TR-N

Protein A goat polyclonal antibody, Texas Red

Specifications

Product Data

• Applications: ELISA, FC, IF, IHC
• Recommended Dilution: Suitable for immunomicroscopy and flow cytometry or FACS analysis as well as other antibody based fluorescent assays requiring lot-to-lot consistency.
  Flow cytometry: 1:2,000 - 1:10,000.
  Immunoflourescence: 1:500 - 1:2,500.
• Host: Goat
• Isotype: IgG
• Clonality: Polyclonal
• Immunogen: Protein A [Staphylococcus aureus]
• Specificity: This antibody detects Protein A.
  Assay by immunoelectrophoresis resulted in a single precipitin arc against purified and partially purified Protein A [Staphylococcus aureus], anti-Goat IgG and anti-Goat Serum.
• Formulation: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2 as buffer, 10 mg/ml Bovine Serum Albumin (BSA) IgG and Protease free as stabilizer, 0.01% (w/v) Sodium Azide as preservative
  Label: Texas Red
  State: Lyophilized Ig fraction
  Label: (TM) Sulfonyl Chloride (Molecular Weight 625 daltons)
  Absorption emission: 596 nm / 620 nm
  Molar radio: 1.5 moles Texas Red(TM) per mole of Goat IgG
• Reconstitution Method: Restore with 2.0 ml of deionized water (or equivalent).
• Concentration: lot specific
• Purification: Multi-step process which includes delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer
• Conjugation: Texas Red
• Storage: Store vial at 2-8° C prior to restoration. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for two weeks at 2-8° C as an undiluted liquid. Dilute only prior to immediate use.
• Stability: Shelf life: One year from despatch.