MIF (103-115) Goat Polyclonal Antibody

CAT#: AP08883PU-N

MIF (103-115) goat polyclonal antibody, Aff - Purified

Product Images

MIF Antibody (0.01ug/ml) staining of Human Thymus lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

• 

  Thymus: Formalin-Fixed, Paraffin-Embedded (FFPE) using MIF Antibody

Specifications

Product Data

• Applications: ELISA, IHC, WB
• Recommended Dilution: ELISA: 1/128000.
  Immunohistochemistry on Paraffin Sections: 2.5 µg/ml.
  Western Blot: 0.01 - 0.03 µg/ml.
• Reactivities: Human, Mouse, Rat, Monkey
• Host: Goat
• Isotype: IgG
• Clonality: Polyclonal
• Immunogen: Synthetic peptide from C-terminus of Human MIF
• Specificity: This antibody recognizes Macrophage Migration Inhibitory Factor (MIF) at aa 103-115.
• Formulation: Tris saline buffer, pH 7.3 containing 0.5% BSA as stabilizer and 0.02% Sodium Azide as preservative
  State: Aff - Purified
  State: Liquid purified IgG fraction
• Concentration: lot specific
• Purification: Immunoaffinity Chromatography
• Conjugation: Unconjugated
• Storage: Store the antibody undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer.
  Avoid repeated freezing and thawing.
• Stability: Shelf life: one year from despatch.
• Gene Name: macrophage migration inhibitory factor (glycosylation-inhibiting factor)
• Database Link:
  Entrez Gene 4282 Human
  UniProt ID P14174
• Background: MIF is a proinflammatory cytokine involved in many inflammatory reactions and disorders. MIF (macrophage migration inhibitory factor) was one of the first cytokines to be discovered and was initially described as a T cell-derived factor that inhibits the random migration of macrophages (Weiser 1989). Recently, MIF was rediscovered as a pituitary hormone that act as the counterregulatory hormone for glucocorticoid action within the immune system (Bernhagen 1993, Mitchell 1995). MIF was released from macrophages and T cells in response to physiological concentrations of glucocorticoids. The secreted MIF counter-regulates the immunosuppressive effects of steroids on immune cell activation and cytokine production (Bucala 1998). MIF plays a critical role in the host control of inflammation and immunity. MIF is involved in both autoimmune disorders and tumorigenesis.
• Synonyms: Macrophage migration inhibitory factor, Phenylpyruvate tautomerase, Glycosylation-inhibiting factor, GLIF, MMIF