MTOR Rabbit Polyclonal Antibody

CAT#: AP08060PU-S

MTOR rabbit polyclonal antibody, Aff - Purified

Product Images

Western Blot analysis of extracts from HeLa. 293 and JK cells using mTOR antibody

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  Immunohistochemical analysis of paraffin-embedded human Lung carcinoma tissue using mTOR Antibody.

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  Immunofluorescence staining of methanol-fixed MCF7 cells using PAK1 antibody (Red).

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  Immunofluorescence staining of methanol-fixed MCF7 cells using mTOR Antibody.

Specifications

Product Data

• Applications: IF, IHC, WB
• Recommended Dilution: Western blot: 1/500-1/1000.
  Immunofluorescence: 1/100-1/200.
  Immunohistochemistry on Paraffin-Embedded Sections: 1/50-1/100.
• Reactivities: Human, Mouse, Rat
• Host: Rabbit
• Clonality: Polyclonal
• Immunogen: The antiserum was produced against synthesized non-phosphopeptide derived from human mTOR around the phosphorylation site of Serine 2448 (T-D-SP-Y-S).
• Specificity: This antibody AP08060PU detects endogenous levels of total mTOR protein.
• Formulation: PBS (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 0.02% Sodium Azide and 50% Glycerol.
  State: Aff - Purified
  State: Liquid purified Ig fraction.
• Concentration: lot specific
• Purification: Immunoaffinity Chromatography using epitope-specific immunogen.
• Conjugation: Unconjugated
• Storage: Store the antibody (in aliquots) at -20°C.
  Avoid repeated freezing and thawing.
• Stability: Shelf life: One year from despatch.
• Gene Name: mechanistic target of rapamycin
• Database Link:
  Entrez Gene 2475 Human
  UniProt ID P42345
• Background: mTOR, or FKBP12 rapamycin associated protein (FRAP), is one of a family of proteins involved in cell cycle progression, DNA recombination, and DNA damage detection. In rat, it is a 289-kDa protein (symbolized RAFT1) with significant homology to the Saccharomyces cerevisiae protein TOR1 and has been shown to associate with the immunophilin FKBP12 in a rapamycin dependent fashion. The FKBP12-rapamycin complex is known to inhibit progression through the G1 cell cycle stage by interfering with mitogenic signaling pathways involved in G1 progression in several cell types, as well as in yeast. The binding of FRAP to FKBP12-rapamycin correlated with the ability of these ligands to inhibit cell cycle progression.
• Synonyms: Mammalian target of rapamycin, TOR, FRAP, FRAP2, RAPT1