SMAD2 pSer467 Rabbit Polyclonal Antibody

CAT#: AP08025PU-S

SMAD2 pSer467 rabbit polyclonal antibody, Aff - Purified

Product Images

Figure 1. Western blot analysis of extract from 293 cells, untreated or treated with PMA (200nM, 30min), using SMAD2 Antibody AP08072PU (Lane 1 and 2) and SMAD2 (Phospho-Ser467) Antibody AP08025PU (Lane 3 and 4).

Specifications

Product Data

• Applications: WB
• Recommended Dilution: Wetern Blot (1/500-1/1000).
• Reactivities: Human, Mouse, Rat
• Host: Rabbit
• Clonality: Polyclonal
• Immunogen: The antiserum was produced against synthesized phosphopeptide derived from human Smad2 around the phosphorylation site of Serine 467 (C-S-S-M-SP).
• Specificity: SMAD2 (Phospho-Ser467) Antibody AP08025PU detects endogenous levels of Smad2 only when phosphorylated at Serine 467.
• Formulation: PBS (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 0.02% Sodium Azide and 50% Glycerol.
  State: Aff - Purified
  State: Liquid purified Ig fraction.
• Concentration: lot specific
• Purification: Immunoaffinity Chromatography: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.
• Conjugation: Unconjugated
• Storage: Store the antibody (in aliquots) at -20°C.
  Avoid repeated freezing and thawing.
• Stability: Shelf life: One year from despatch.
• Gene Name: SMAD family member 2
• Database Link:
  Entrez Gene 4087 Human
  UniProt ID Q15796
• Synonyms: SMAD family member 2, SMAD-2, SMAD 2, MADH2, MAD homolog 2, MADR2, Mad-related protein 2, JV18-1