SMAD1 pSer465 Rabbit Polyclonal Antibody

CAT#: AP08024PU-N

SMAD1 pSer465 rabbit polyclonal antibody, Aff - Purified

Product Images

Figure 1. Western blot analysis of extract from HeLa cells untreated or treated with PMA using SMAD1 Antibody (Phospho-Ser465)

Specifications

Product Data

• Applications: WB
• Recommended Dilution: Wetern Blot (1/500-1/1000).
• Reactivities: Human, Mouse, Rat
• Host: Rabbit
• Clonality: Polyclonal
• Immunogen: The antiserum was produced against synthesized phosphopeptide derived from Human Smad1 around the phosphorylation site of Serine 465 (I-S-S-V-SP).
• Specificity: SMAD1 (Phospho-Ser465) Antibody AP08024PU detects endogenous levels of Smad1 only when phosphorylated at Serine 465.
• Formulation: PBS (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 0.02% Sodium Azide and 50% Glycerol.
  State: Aff - Purified
  State: Liquid purified Ig fraction.
• Concentration: lot specific
• Purification: Immunoaffinity Chromatography: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.
• Conjugation: Unconjugated
• Storage: Store the antibody (in aliquots) at -20°C.
  Avoid repeated freezing and thawing.
• Stability: Shelf life: One year from despatch.
• Gene Name: SMAD family member 1
• Database Link:
  Entrez Gene 4086 Human
  UniProt ID Q15797
• Background: SMAD proteins are signal transducers and transcriptional modulators that mediate multiple signaling pathways. SMAD1, as a transcriptional modulator, is activated by BMP (Bone Morphogenetic Protein) type 1 receptor kinase (it is a receptor-regulated SMAD or R-SMAD). BMPs are involved in a range of biological activities including cell growth, apoptosis, morphogenesis, development and immune responses. SMAD proteins have been implicated as downstream effectors of TGF beta/BMP signaling. In response to BMP ligands, SMAD1 can be phosphorylated (other sites besides the most prominent of S206, are S187, S195, and S214). S-206 is phosphorylated by ERK in response to mitogenic growth factors, or by recombinant ERK in vitro; this can be tested by treating cells with EGF or in cancer cells where Ras is activated. The phosphorylated form of this protein forms a complex with SMAD4, which is important for its function in the transcription regulation. This protein is also a target for SMADspecific E3 ubiquitin ligases, such as SMURF1 and SMURF2, and undergoes ubiquitination and proteasome-mediated degradation.
• Synonyms: SMAD family member 1, SMAD-1, SMAD 1, MADH1, MAD homolog 1, MADR1, Mad-related protein 1, BSP1, BSP-1, JV4-1
• Note: Molecular Weight: 55 kDa