p53 (TP53) pSer6 Rabbit Polyclonal Antibody

CAT#: AP08001PU-S

p53 (TP53) pSer6 rabbit polyclonal antibody, Aff - Purified

Product Images

Figure 1. Western blot analysis of extract from HT-29 cells untreated or treated with UV (20 min), using p53 antibody AP08045PU (Lane 1 and 2) and p53 (pSer6) antibody AP08001PU-S (Lane 3 and 4).

Specifications

Product Data

• Applications: IHC, WB
• Recommended Dilution: Western blot (1/500-1/1000).
  Immunohistochemistry on Paraffin Sections (1/50-1/100).
• Reactivities: Human, Mouse
• Host: Rabbit
• Clonality: Polyclonal
• Immunogen: Peptide sequence around phosphorylation site of serine 6 (P-Q-S(p)-D-P) derived from Human p53
• Specificity: This antibody (p53 pSer6) detects endogenous levels of p53 only when phosphorylated at serine 6.
• Formulation: PBS (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 0.02% Sodium Azide and 50% Glycerol.
  State: Aff - Purified
  State: Liquid purified Ig fraction.
• Concentration: lot specific
• Purification: Immunoaffinity Chromatography: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.
• Conjugation: Unconjugated
• Storage: Store undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer.
  Avoid repeated freezing and thawing.
• Stability: Shelf life: one year from despatch.
• Predicted Protein Size: 53 kDa
• Gene Name: tumor protein p53
• Database Link:
  Entrez Gene 7157 Human
  UniProt ID P04637
• Synonyms: Cellular tumor antigen p53, Tumor suppressor p53, Phosphoprotein p53, NY-CO-13