N Cadherin (CDH2) Rabbit Polyclonal Antibody

CAT#: AP06797PU-N

N Cadherin (CDH2) rabbit polyclonal antibody, Aff - Purified

Product Images

Western blot (WB) analysis of N-Cadherin antibody at 1/500 dilution Lane 1: A549 whole cell lysate Lane 2: Mouse brain tissue lysate Lane 3: Rat brain tissue lysate

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  Immunohistochemistry (IHC) analyzes of N-cadherin antibody in paraffin-embedded human brain tissue.

Specifications

Product Data

• Applications: ELISA, IHC, WB
• Recommended Dilution: Western blot: 1/500-1/1000.
  Immunohistochemistry on paraffin sections: 1/50-1/200.
  Immunofluorescence: 1/50-1/200.
• Reactivities: Human, Mouse, Rat
• Host: Rabbit
• Clonality: Polyclonal
• Immunogen: Synthetic peptide, corresponding to amino acids 710-760 of Human N-Cadherin.
• Specificity: This antibody detects endogenous levels of N-cadherin protein.
  (region surrounding Trp745)
• Formulation: Phosphate buffered saline (PBS), pH 7.2.
  State: Aff - Purified
  State: Liquid purified Ig fraction
  Preservative: 15 mM sodium azide
• Concentration: 1.0 mg/ml
• Purification: Affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE)
• Conjugation: Unconjugated
• Storage: Store undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer.
  Avoid repeated freezing and thawing.
• Stability: Shelf life: one year from despatch.
• Predicted Protein Size: ~ 100, 140 kDa
• Gene Name: cadherin 2
• Database Link:
  Entrez Gene 1000 Human
  UniProt ID P19022
• Background: Cadherins comprise a family of Ca2+-dependent adhesion molecules that function to mediate cell-cell binding critical to the maintenance of tissue structure and morphogenesis. The classical cadherins, E-, N- and P-cadherin, consist of large extracellular domains characterized by a series of five homologous NH2-terminal repeats. The most distal of these cadherins is thought to be responsible for binding specificity, transmembrane domains and carboxyterminal intracellular domains. The relatively short intracellular domains interact with a variety of cytoplasmic proteins, such as β-catenin, to regulate cadherin function. Members of this family of adhesion proteins include rat cadherin K (and its human homolog, cadherin-6), R-cadherin, B-cadherin, E/P-cadherin and cadherin-5.
• Synonyms: CDH2, CDHN, NCAD, Neural cadherin, CDw325, Cadherin N, N-Cadherin