beta Catenin (CTNNB1) Rabbit Polyclonal Antibody

CAT#: AP06683PU-N

beta Catenin (CTNNB1) rabbit polyclonal antibody, Aff - Purified

Product Images

Western blot (WB) analysis of Catenin-β antibody at 1/500 dilution Lane 1:HEK293T whole cell lysate Lane 2:Raw264.7 whole cell lysate Lane 3:Rat kideny tissue lysate

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  Immunohistochemistry (IHC) analyzes of Catenin-β1 antibody in paraffin-embedded human breast carcinoma tissue.

Specifications

Product Data

• Applications: IF, IHC, WB
• Recommended Dilution: Western blot: 1/500-1/1000.
  Immunohistochemistry on paraffin sections: 1/50-1/200.
  Immunofluorescence: 1/50-1/200.
• Reactivities: Human, Mouse, Rat
• Host: Rabbit
• Clonality: Polyclonal
• Immunogen: Synthetic peptide, corresponding to amino acids 640-690 of Human Catenin-β.
• Specificity: This antibody detects endogenous levels of Catenin-beta1 protein.
  (region surrounding Phe660)
• Formulation: Phosphate buffered saline (PBS), pH 7.2.
  State: Aff - Purified
  State: Liquid purified Ig fraction
  Preservative: 15 mM sodium azide
• Concentration: 1.0 mg/ml
• Purification: Affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE)
• Conjugation: Unconjugated
• Storage: Store undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer.
  Avoid repeated freezing and thawing.
• Stability: Shelf life: one year from despatch.
• Predicted Protein Size: ~ 92 kDa
• Gene Name: catenin beta 1
• Database Link:
  Entrez Gene 1499 Human
  UniProt ID P35222
• Background: β-Catenin is a key downstream effector in the Wnt signaling pathway. It is implicated in two major biological processes in vertebrates: early embryonic development and tumorigenesis. CK1 phosphorylates β-catenin at Ser45. This phosphorylation event primes β-catenin for subsequent phosphorylation by GSK-3β. GSK-3β destabilizes β-catenin by phosphorylating it at Ser33, Ser37, and Thr41. Mutations at these sites result in the stabilization of β-catenin protein levels and have been found in many tumor cell lines.
• Synonyms: CTNNB1, CTNNB, Beta-catenin