CEBP Beta (CEBPB) Rabbit Polyclonal Antibody

CAT#: AP06676PU-N

CEBP Beta (CEBPB) rabbit polyclonal antibody, Aff - Purified

Product Images

Western blot (WB) analysis of C/EBP-β antibody at 1/500 dilution Lane 1:HEK293T whole cell lysate Lane 2:NIH-3T3 whole cell lysate Lane 3:H9C2 whole cell lysate

Specifications

Product Data

• Applications: IHC, WB
• Recommended Dilution: Western blot: 1/500-1/1000.
  Immunohistochemistry on paraffin sections: 1/50-1/200.
• Reactivities: Human, Mouse
• Host: Rabbit
• Clonality: Polyclonal
• Immunogen: Synthetic peptide, corresponding to amino acids 220-270 of Human C/EBP-β.
• Specificity: This antibody detects endogenous levels of C/EBP-β protein.
  (region surrounding Val259)
• Formulation: Phosphate buffered saline (PBS), pH~7.2
  State: Aff - Purified
  State: Liquid purified Ig fraction (>95% by SDS-PAGE)
  Preservative: 15mM Sodium Azide
• Concentration: 1.0 mg/ml
• Conjugation: Unconjugated
• Storage: Store undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer.
  Avoid repeated freezing and thawing.
• Stability: Shelf life: one year from despatch.
• Predicted Protein Size: ~ 36 kDa
• Gene Name: CCAAT/enhancer binding protein beta
• Database Link:
  Entrez Gene 1051 Human
  UniProt ID P17676
• Background: C/EBP β is a member of the C/EBP transcription factor family. The C/EBP β gene encodes several isoforms, which have truncated transcription activation domains by the alternative translational initiation at multipe AUG start sites. Initiation of translation at the in-frame AUGs forms four C/EBP β isoforms. C/EBP β is also known as interleukin 6-dependent DNA-binding protein (IL6DBP), liver activator protein(LAP) or liver-enriched transcriptional activator protein transcription factor 5 (TCF5). C/EBP β contributes to the regulation of the acute phase response in hepatocytes. Stat3 has an important function in IL-6-mediated transcription of the C/EBP β gene that has direct implication for acute phase response in liver cells.The 35 kDa C/EBP β form requires phosphorylation for its DNA binding ability, and increased binding of C/EBP β isoforms during acute-phase reaction occurs through its upregulation and structural modification.
• Synonyms: C/EBP beta, TCF5, LAP, NF-IL6