BMX Rabbit Polyclonal Antibody

CAT#: AP06522PU-N

BMX rabbit polyclonal antibody, Aff - Purified

Product Images

Western blot (WB) analysis of ETK antibody in extracts from A549 cells.

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  Immunohistochemistry (IHC) analysis of ETK antibody in paraffin-embedded human skin tissue.

Specifications

Product Data

• Applications: IF, IHC, WB
• Recommended Dilution: Western blot: 1/500-1/1000.
  Immunohistochemistry on paraffin sections: 1/50-1/200.
  Immunofluorescence: 1/50-1/200.
• Reactivities: Human, Mouse
• Host: Rabbit
• Clonality: Polyclonal
• Immunogen: Synthetic peptide, corresponding to amino acids N-terminus of Human ETK.
• Specificity: This antibody detects endogenous levels of ETK protein.
  (region surrounding Lys35)
• Formulation: Phosphate buffered saline (PBS), pH 7.2.
  State: Aff - Purified
  State: Liquid purified Ig fraction
  Preservative: 15 mM sodium azide
• Concentration: 1.0 mg/ml
• Purification: Affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE)
• Conjugation: Unconjugated
• Storage: Store undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer.
  Avoid repeated freezing and thawing.
• Stability: Shelf life: one year from despatch.
• Predicted Protein Size: ~ 78 kDa
• Gene Name: BMX non-receptor tyrosine kinase
• Database Link:
  Entrez Gene 12169 MouseEntrez Gene 660 Human
  UniProt ID P51813
• Background: Etk, also known as BMX, is a member of the Bruton's tyrosine kinase (Btk) family (1). It is expressed in a variety of hematopoietic, epithelial and endothelial cells. Etk, like other Btk family members, contains a pleckstrin homology (PH) domain and Src homology SH3 and SH2 domains. It participates in multiple signal transduction pathways (2). Phosphorylation of Tyr566 by Src kinase is required for activation of Etk in vivo (3). In endothelial and epithelial cells, Etk is regulated by FAK through phosphorylation at Tyr40 (4).
• Synonyms: NTK38, ETK

Reference Data

• Protein Families: Druggable Genome, Protein Kinase