Tyrosine Hydroxylase (TH) Rabbit Polyclonal Antibody

CAT#: AP06420PU-N

Tyrosine Hydroxylase (TH) rabbit polyclonal antibody, Aff - Purified

Product Images

Western blot (WB) analysis of Tyrosine Hydroxylase antibody in extracts from 293 cells treated with UV 15' or COS7 cells treated with Heat shock.

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  Immunohistochemistry (IHC) analyzes of Tyrosine Hydroxylase antibody in paraffin-embedded human breast carcinoma tissue.

Specifications

Product Data

• Applications: IHC, WB
• Recommended Dilution: Western blot: 1/500-1/1000.
  Immunohistochemistry on paraffin sections: 1/50-1/200.
• Reactivities: Human, Mouse, Rat
• Host: Rabbit
• Clonality: Polyclonal
• Immunogen: Synthetic peptide, corresponding to the N-terminus of Human TH.
• Specificity: This antibody detects endogenous levels of Tyrosine Hydroxylase protein.
  (region surrounding Gln25)
• Formulation: Phosphate buffered saline (PBS), pH 7.2.
  State: Aff - Purified
  State: Liquid purified Ig fraction
  Preservative: 0.05% sodium azide
• Concentration: 1.0 mg/ml
• Purification: Affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE)
• Conjugation: Unconjugated
• Storage: Store undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer.
  Avoid repeated freezing and thawing.
• Stability: Shelf life: one year from despatch.
• Predicted Protein Size: ~ 58 kDa
• Gene Name: tyrosine hydroxylase
• Database Link:
  Entrez Gene 7054 Human
  UniProt ID P07101
• Background: The enzyme tyrosine hydroxylase (TH), also designated tyrosine 3-monooxygenase (TY3H), catalyzes the conversion of tyrosine to L-dopa, which is the rate limiting step in the biosynthesis of catecholamines such as dopamine, adrenalin and noradrenalin. TH is thought to play a role in the pathogenesis of Parkinson’s disease, which is associated with reduced dopamine levels. Two transcription factor binding sites in the proximal region of the TH gene, the TPA-responsive element (TRE) and the c-AMP responsive element (CRE), have been implicated in the complex regulation of the TH gene. TH is also known to be upregulated by the glia maturation factor (GMF), a Cdc 10/SWI6 motif-containing protein called V-1, and a variety of additional compounds.
• Synonyms: Tyrosine 3-hydroxylase, TYH