CEBP Alpha (CEBPA) Rabbit Polyclonal Antibody

CAT#: AP06373PU-N

CEBP Alpha (CEBPA) rabbit polyclonal antibody, Aff - Purified

Product Images

Western blot (WB) analysis of C/EBPA antibody in extracts from 293 cells treated with Insulin 0.01U/ml 15'.

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  Immunohistochemistry (IHC) analyzes of C/EBPA antibody in paraffin-embedded human lung carcinoma tissue.

Specifications

Product Data

• Applications: IHC, WB
• Recommended Dilution: Western blot: 1/500-1/1000.
  Immunohistochemistry on Paraffin Sections: 1/50-1/200.
• Reactivities: Human, Mouse, Rat
• Host: Rabbit
• Clonality: Polyclonal
• Immunogen: Synthetic peptide, corresponding to amino acids N-terminus of Human C/EBP-α.
• Specificity: This antibody detects endogenous levels of C/EBP-alpha protein.
  (region surrounding Met15)
• Formulation: Phosphate buffered saline (PBS), pH~7.2
  State: Aff - Purified
  State: Liquid purified Ig fraction (> 95% pure by SDS-PAGE).
  Preservative: 0.05% sodium azide
• Concentration: 1.0 mg/ml
• Purification: Affinity Chromatography using epitope-specific immunogen.
• Conjugation: Unconjugated
• Storage: Store the antibody undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer.
  Avoid repeated freezing and thawing.
  
• Stability: Shelf life: One year from despatch.
• Predicted Protein Size: ~30.43 kDa
• Gene Name: CCAAT/enhancer binding protein alpha
• Database Link:
  Entrez Gene 1050 Human
  UniProt ID P49715
• Background: The protein encoded by this intronless gene is a bZIP transcription factor which can bind as a homodimer to certain DNA regulatory regions. It can also form heterodimers with the related proteins CEBP alpha, CEBP delta, and CEBP gamma. The encoded protein is important in the regulation of genes involved in immune and inflammatory responses and has been shown to bind to the IL1 response element in the IL6 gene, as well as to regulatory regions of several acute-phase and cytokine genes. In addition, the encoded protein can bind the promoter and upstream element and stimulate the expression of the collagen type I gene.
• Synonyms: C/EBP alpha