Cytokeratin 20 (KRT20) Rabbit Polyclonal Antibody

CAT#: AP06202PU-N

Cytokeratin 20 (KRT20) rabbit polyclonal antibody, Aff - Purified

Product Images

Western blot (WB) analysis of Cytokeratin 20 antibody at 1/500 dilution Lane 1: A549 whole cell lysate Lane 2: SP2/0 whole cell lysate Lane 3: Rat kidney tissue lysate Lane 4: Mouse kidney tissue lysate

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  Immunohistochemistry (IHC) analyzes of Keratin 20 antibody in paraffin-embedded human breast carcinoma tissue.

Specifications

Product Data

• Applications: IF, IHC, WB
• Recommended Dilution: Western blot: 1/500-1/1000.
  Immunofluorescence: 1/50-1/200.
  Immunohistochemistry on Paraffin Sections: 1/50-1/200.
• Reactivities: Human, Mouse, Rat
• Host: Rabbit
• Clonality: Polyclonal
• Immunogen: Synthetic peptide, corresponding to amino acids C-terminus of Human Cytokeratin 20.
• Specificity: This antibody detects endogenous levels of Cytokeratin 20 protein.
  (region surrounding Asp409)
• Formulation: Phosphate buffered saline (PBS), pH~7.2
  State: Aff - Purified
  State: Liquid purified Ig fraction (> 95% pure by SDS-PAGE).
  Preservative: 0.05% Sodium Azide
• Concentration: 1.0 mg/ml
• Purification: Affinity Chromatography using epitope-specific immunogen.
• Conjugation: Unconjugated
• Storage: Store undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer.
  Avoid repeated freezing and thawing.
• Stability: Shelf life: One year from despatch.
• Predicted Protein Size: ~48 kDa
• Gene Name: keratin 20
• Database Link:
  Entrez Gene 54474 Human
  UniProt ID P35900
• Background: Cytokeratins play a critical role in differentiation and tissue specialization and function to maintain the overall structural integrity of epi-thelial cells. The a-helical coiled-coil dimers associate laterally end-to-end to form 10 nm diameter filaments. Cytokeratins are useful markers of tissue differentiation, and in addition, they aid in the characterization of malignant tumors. Cytokeratin 20 is abundantly expressed in goblet cells and enterocytes of the gastrointestinal tract, and Cytokeratin 20 is a useful marker of pancreatic and colorectal cancer. Cytokeratin 20 is also helpful in distinguishing different types of highly related carcinomas, such as renal oncocytomas from renal cell carcinomas.
• Synonyms: Cytokeratin 20, Keratin-20, KRT20, CK20, K20, Protein IT