G3BP (G3BP1) Rabbit Polyclonal Antibody

CAT#: AP06127PU-N

G3BP (G3BP1) rabbit polyclonal antibody, Aff - Purified

Product Images

Western blot (WB) analysis of G3BP-1 antibody at 1/500 dilution Lane 1:A549 whole cell lysate Lane 2:Mouse heart tissue lysate Lane 3:Rat heart tissue lysate

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  Immunohistochemistry (IHC) analyzes of G3BO-1 antibody in paraffin-embedded human lymphoma tissue.

Specifications

Product Data

• Applications: IHC, WB
• Recommended Dilution: Western blot: 1/500-1/1000.
  Immunohistochemistry on paraffin sections: 1/50-1/200.
• Reactivities: Human, Mouse
• Host: Rabbit
• Clonality: Polyclonal
• Immunogen: Synthetic peptide, corresponding to amino acids 196-246 of Human G3BP-1.
• Specificity: This antibody detects endogenous levels of G3BP-1 protein.
  (region surrounding Asp226)
• Formulation: Phosphate buffered saline (PBS), pH 7.2.
  State: Aff - Purified
  State: Liquid purified Ig fraction (> 95% pure by SDS-PAGE)
  Preservative: 0.05% Sodium azide
• Concentration: 1.0 mg/ml
• Purification: Affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE)
• Conjugation: Unconjugated
• Storage:

  Store undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer.
  Avoid repeated freezing and thawing.

• Stability: Shelf life: one year from despatch.

• Predicted Protein Size: ~ 50 kDa
• Gene Name: G3BP stress granule assembly factor 1
• Database Link:
  Entrez Gene 10146 Human
  UniProt ID Q13283
• Background: G3BP is one of the DNA-unwinding enzymes which prefers partially unwound 3'-tailed substrates and can also unwind partial RNA/DNA and RNA/RNA duplexes in an ATP-dependent fashion. This enzyme is a member of the heterogeneous nuclear RNA-binding proteins and is also an element of the Ras signal transduction pathway. It binds specifically to the Ras-GTPase-activating protein by associating with its SH3 domain. Several alternatively spliced transcript variants of this gene have been described, but the full-length nature of some of these variants has not been determined.
• Synonyms: G3BP-1, hDH VIII