Estrogen Receptor 1 (ESR1) Rabbit Polyclonal Antibody

CAT#: AP06109PU-N

Estrogen Receptor 1 (ESR1) rabbit polyclonal antibody, Aff - Purified

Product Images

Western blot (WB) analysis of Estrogen Receptor-a antibody in extracts from MCF7 cell treated with Estradiol.

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  Immunohistochemical analysis in paraffin-embedded human breast carcinoma tissue using Estrogen receptor alpha antibody.

Specifications

Product Data

• Applications: IHC, WB
• Recommended Dilution:

  Western blot: 1/500-1/1000.
  Immunohistochemistry on Paraffin sections: 1/50-1/200.

• Reactivities: Human, Mouse, Rat
• Host: Rabbit
• Clonality: Polyclonal
• Immunogen: Synthetic peptide, corresponding to amino acids 61-110 of Human ERα.
• Specificity: This antibody detects endogenous levels of Estrogen receptor alpha (region surrounding Leu100).
• Formulation: Phosphate buffered saline (PBS), pH 7.2.
  State: Aff - Purified
  State: Liquid purified Ig fraction
  Preservative: 0.05% Sodium azide
• Concentration: 1.0 mg/ml
• Purification: Affinity chromatography using epitope-specific immunogen (> 95% pure by SDS-PAGE).
• Conjugation: Unconjugated
• Storage:

  Store undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer.
  Avoid repeated freezing and thawing.

• Stability: Shelf life: one year from despatch.

• Predicted Protein Size: ~66 kDa
• Gene Name: estrogen receptor 1
• Database Link:
  Entrez Gene 2099 Human
  UniProt ID P03372
• Background: Estrogen receptors (ER) are members of the steroid/thyroid hormone receptor superfamily of ligand-activated transcription factors. Estrogen receptors, including ERα and ERβ, contain DNA binding and ligand binding domains and are critically involved in regulating the normal function of reproductive tissues. ERα and ERβ have been shown to be differentially activated by various ligands. Receptor-ligand interactions trigger a cascade of events, including dissociation from heat shock proteins, receptor dimerization, phosphorylation and the association of the hormone activated receptor with specific regulatory elements in target genes. Evidence suggests that ERα and ERβ may be regulated by distinct mechanisms even though they share many functional characteristics.
• Synonyms: ER alpha, Estradiol receptor, ESR1, ESR, NR3A1