ERK1 (MAPK3) Rabbit Polyclonal Antibody

CAT#: AP06107PU-N

ERK1 (MAPK3) rabbit polyclonal antibody, Aff - Purified

Product Images

Western blot (WB) in extracts from COLO205 cells using MAPK3 / ERK1 antibody.

• 

  Immunohistochemical (IHC) analysis in paraffin-embedded human breast carcinoma tissue using MAPK3 / ERK1 antibody.

Specifications

Product Data

• Applications: IHC, WB
• Recommended Dilution:

  Western blot: 1/500-1/1000.
  Immunohistochemistry on Paraffin sections: 1/50-1/200.

• Reactivities: Human, Mouse, Rat
• Host: Rabbit
• Clonality: Polyclonal
• Immunogen: Synthetic peptide, corresponding to amino acids 320-370 of Human ERK1.
• Specificity: This antibody detects endogenous levels of MAPK3 / ERK1 protein (region surrounding Leu352).
• Formulation: Phosphate buffered saline (PBS), pH 7.2.
  State: Aff - Purified
  State: Liquid purified Ig fraction (> 95% pure by SDS-PAGE)
  Preservative: 0.05% Sodium Azide
• Concentration: 1.0 mg/ml
• Purification: Affinity Chromatography using epitope-specific immunogen
• Conjugation: Unconjugated
• Storage:

  Store undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer.
  Avoid repeated freezing and thawing.

• Stability: Shelf life: one year from despatch.

• Predicted Protein Size: ~42,44 kDa
• Gene Name: mitogen-activated protein kinase 3
• Database Link:
  Entrez Gene 5595 Human
  UniProt ID P27361
• Background: The activation of signal transduction pathways by growth factors, hormones and neurotransmitters is mediated through two closely related MAP kinases, p44 and p42, designated extracellular-signal related kinase 1 (ERK 1) and ERK 2, respectively. ERK proteins are regulated by dual phosphorylation at specific tyrosine and Threonine sites mapping within a characteristic Thr- Glu-Tyr motif. Phosphorylation at both the Thr and Tyr residues is required for full enzymatic activation. In response to activation, MAP kinases phos-phorylate downstream components on Serine and Threonine. Upstream MAP kinase regulators include MAP kinase kinase (MEK), MEK kinase and Raf-1. The ERK family has three additional members: ERK 3, ERK 5 and ERK 6.
• Synonyms: MAP kinase 3, MAPK 3, ERK-1, ERT2, p44-MAPK, p44-ERK1, PRKM3