Collagen IV (COL4A1) Rabbit Polyclonal Antibody

CAT#: AP06069PU-N

Collagen IV (COL4A1) rabbit polyclonal antibody, Aff - Purified

Product Images

Western blot (WB) analysis of COL4A1 antibody at 1/500 dilution Lane 1:MCF-7 whole cell lysate Lane 2:Mouse liver tissue lysate Lane 3:Rat liver tissue lysate

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  Immunohistochemistry analyzes of Collagen IV antibody in paraffin-embedded human breast carcinoma tissue.

Specifications

Product Data

• Applications: ELISA, IHC, WB
• Recommended Dilution: Western blot: 1/500-1/1000.
  Immunohistochemistry on Paraffin Sections: 1/50-1/200.
• Reactivities: Human, Mouse
• Host: Rabbit
• Clonality: Polyclonal
• Immunogen: Synthetic peptide, corresponding to amino acids 11-60 of Human COL4A1.
• Specificity: This antibody detects endogenous levels of Collagen IV protein.
  (region surrounding Gly46)
• Formulation: Phosphate buffered saline (PBS), pH~7.2
  State: Aff - Purified
  State: Liquid purified Ig fraction (> 95% pure by SDS-PAGE)
  Preservative: 0.05% Sodium Azide
• Concentration: 1.0 mg/ml
• Purification: Affinity-chromatography using epitope-specific immunogen
• Conjugation: Unconjugated
• Storage: Store undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer.
  Avoid repeated freezing and thawing.
• Stability: Shelf life: One year from despatch.
• Predicted Protein Size: ~ 160 kDa
• Gene Name: collagen type IV alpha 1 chain
• Database Link:
  Entrez Gene 1282 Human
  UniProt ID P02462
• Background: The extensive family of COL gene products (collagens) is composed of several chain types, including fibril-forming interstitial Collagens (Types I, II, III and V) and basement membrane Collagens (Type IV), each type containing multiple isoforms. Collagens are fibrous, extracellular matrix proteins with high tensile strength and are the major components of connective tissue, such as tendons and cartilage. All collagens contain a triple helix domain and frequently show lateral self-association in order to form complex connective tissues. Several collagens also play a role in cell adhesion, important for maintaining normal tissue architecture and function.
• Synonyms: COL4A1