c-Jun (JUN) Rabbit Polyclonal Antibody

CAT#: AP06054PU-N

c-Jun (JUN) rabbit polyclonal antibody, Aff - Purified

Product Images

Western blot analysis of AP-1 / c-Jun Antibody at 1/500 dilution in HeLa cell lysate (Lane 1), Mouse kidney tissue lysate (Lane 2) and Rat brain tissue lysate (Lane 3).

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  Immunohistochemistry analysis of AP-1 / c-Jun Antibody in paraffin-embedded human breast carcinoma tissue.

Specifications

Product Data

• Applications: ELISA, IHC, WB
• Recommended Dilution: ELISA: 1/10000-1/20000.
  Western blot: 1/500-1/1000.
  Immunochemistry on Paraffin Sections: 1/50-1/200.
• Reactivities: Human, Mouse, Rat
• Host: Rabbit
• Clonality: Polyclonal
• Immunogen: Synthetic peptide, corresponding to amino acids 201-250 of Human c-Jun.
• Specificity: The antibody detects endogenous levels of c-Jun protein (region surrounding Pro233).
• Formulation: PBS, pH~7.2
  State: Aff - Purified
  State: Liquid purified Ig fraction (> 95% pure by SDS-PAGE)
  Preservative: 0.05% Sodium Azide
• Concentration: 1.0 mg/ml
• Purification: Affinity-chromatography using epitope-specific immunogen
• Conjugation: Unconjugated
• Storage:

  Store undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer.
  Avoid repeated freezing and thawing.

• Stability: Shelf life: one year from despatch.

• Predicted Protein Size: ~36, 43, 48 kDa
• Gene Name: Jun proto-oncogene, AP-1 transcription factor subunit
• Database Link:
  Entrez Gene 3725 Human
  UniProt ID P05412
• Background: Genes belonging to the Jun and Fos oncogene families encode nuclear proteins that are found to be associated with a number of transcriptional complexes. The c-Jun protein is a major component of the transcription factor AP-1, originally shown to mediate phorbol ester tumor promoter (TPA)-induced expression of responsive genes through the TPA- response element (TRE). The Jun proteins form homo- and heterodimers which bind the TRE, while Fos proteins are active only as heterodimers with any of the Jun proteins. Fos/Jun heterodimers have a much higher affinity for the TRE than Jun homodimers. Ha-Ras augments c-Jun activity and stimulates phosphorylation of its activation domain. An inhibitor of Fos/Jun function, termed IP-1, associates with Fos and Jun and is inactivated upon phosphorylation induced by the cAMP-dependent protein kinase A (PKA).
• Synonyms: Transcription factor AP1