BAX Rabbit Polyclonal Antibody

CAT#: AP06027PU-N

BAX rabbit polyclonal antibody, Aff - Purified

Product Images

Western blot (WB) analysis of Bax antibody at 1/500 dilution Lane 1:MCF-7 cell lysate Lane 2:Raw264.7 cell lysate Lane 3:Rat brain tissue lysate

• 

  Immunohistochemical analysis in paraffin-embedded human brain tissue using Bax antibody.

Specifications

Product Data

• Applications: IHC, IP, WB
• Recommended Dilution: Western blot: 1/500-1/1000.
  Immunohistochemistry on Paraffin sections: 1/50-1/200.
  Immunoprecipitation: 1/50-1/200.
• Reactivities: Human, Mouse, Rat
• Host: Rabbit
• Clonality: Polyclonal
• Immunogen: Synthetic peptide, corresponding to the N-terminus of Human Bax.
• Specificity: This antibody detects endogenous levels of Bax-α/β/δ/ε/σ protein (region surrounding Ser4).
• Formulation: Phosphate buffered saline (PBS), pH 7.2
  State: Aff - Purified
  State: Liquid purified Ig fraction
  Preservative: 0.05% Sodium azide
• Concentration: 1.0 mg/ml
• Purification: Affinity chromatography using epitope-specific immunogen (> 95% oure by SDS-PAGE)
• Conjugation: Unconjugated
• Storage:

  Store undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer.
  Avoid repeated freezing and thawing.

• Stability: Shelf life: one year from despatch.

• Predicted Protein Size: ~21 kDa
• Gene Name: BCL2 associated X protein
• Database Link:
  Entrez Gene 581 Human
  UniProt ID Q07812
• Background: The Bcl-2 gene was isolated at the chromosomal breakpoint of t-bearing follicular B cell lymphomas. Bcl-2 blocks cell death following a variety of stimuli and confers a death-sparing effect to certain hematopoietic cell lines following growth factor withdrawal. Bcl-2 is localized to outer mitochondrial membranes and endoplasmic reticulum as well as nuclear membranes. A related protein, designated Bax p21 (for Bcl-associated X protein), has extensive amino acid homology with Bcl-2 and both homodimerizes and forms heterodimers with Bcl-2. Overexpression of Bax accelerates apoptotic death induced by cytokine deprivation in an IL-3 dependent cell line, and Bax also counters the death repressor activity of Bcl-2.
• Synonyms: Apoptosis regulator BAX, BCL2L4, Bcl2-L-4