ASK1 (MAP3K5) Rabbit Polyclonal Antibody

CAT#: AP06017PU-N

ASK1 (MAP3K5) rabbit polyclonal antibody, Aff - Purified

Product Images

Western blot (WB) analysis of ASK1 antibody at 1/500 dilution Lane 1:Hela whole cell lysate Lane 2:SP2/0 whole cell lysate Lane 3:H9C2 whole cell lysate

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  Immunohistochemistry (IHC) analyzes of ASK1 antibody in paraffin-embedded human breast carcinoma tissue.

Specifications

Product Data

• Applications: ELISA, IHC, WB
• Recommended Dilution: Western blot: 1/500-1/1000.
  Immunohistochemistry on paraffin sections: 1/50-1/200.
• Reactivities: Human
• Host: Rabbit
• Clonality: Polyclonal
• Immunogen: Synthetic peptide, corresponding to amino acids 43-90 of Human ASK1.
• Specificity: This antibody detects endogenous levels of MEKK5 / ASK1 protein (region surrounding Thr77).
• Formulation: Phosphate buffered saline (PBS), pH 7.2
  State: Aff - Purified
  State: Liquid purified Ig fraction (> 95% pure by SDS-PAGE)
  Preservative: 0.05% Sodium azide
• Concentration: 1.0 mg/ml
• Purification: Affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE)
• Conjugation: Unconjugated
• Storage:

  Store undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer.
  Avoid repeated freezing and thawing.

• Stability: Shelf life: one year from despatch.

• Predicted Protein Size: ~ 155 kDa
• Gene Name: mitogen-activated protein kinase kinase kinase 5
• Database Link:
  Entrez Gene 4217 Human
  UniProt ID Q99683
• Background: Mitogen-activated protein (MAP) kinase cascades are activated by various extracellular stimuli including growth factors. The MEK kinases (also called MAP kinase kinase kinases) phosphorylate and activate the MAP kinases, including ERK, JNK and p38. The MEK kinases characterized to date include Raf-1, Raf-B, MOS, MEK kinase-1, MEK kinase-2, MEK kinase-3, MEK kinase-4 and ASK 1 (also designated MEK kinase-5). MEK kinase-1 has been shown to phosphorylate MEK-1 via a Raf-independent pathway. Evidence suggests that MEK-3 is preferentially activated by MEK kinase-3 and that MEK-4 is activated by both MEK kinase-2 and MEK kinase-3. MEK kinase-4 has been shown to specifically activate the JNK pathway. ASK 1 activates both MEK-4 and MEK-3/MEK-6 pathways.
• Synonyms: MAPK/ERK kinase kinase 5, MAPKKK5, MAP3K5