Androgen Receptor (AR) Rabbit Polyclonal Antibody

CAT#: AP06010PU-N

Androgen Receptor (AR) rabbit polyclonal antibody, Aff - Purified

Product Images

Immunohistochemical analysis using Androgen receptor antibody in Paraffin-embedded human prostate carcinoma tissue.

Specifications

Product Data

• Applications: ELISA, IHC
• Recommended Dilution: Immunohistochemistry on Paraffin sections: 1/50-1/200.
• Reactivities: Human
• Host: Rabbit
• Clonality: Polyclonal
• Immunogen: Synthetic peptide, corresponding to amino acids 171-220 of Human AR.
• Specificity: This antibody detects endogenous levels of Androgen receptor protein (region surrounding Gly207).
• Formulation: Phosphate buffered saline (PBS), pH 7.2
  State: Aff - Purified
  State: Liquid purified Ig fraction
  Preservative: 0.05% Sodium azide
• Concentration: 1.0 mg/ml
• Purification: Affinity chromatography using epitope-specific immunogen (> 95% pure; by SDS-PAGE)
• Conjugation: Unconjugated
• Storage:

  Store undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer.
  Avoid repeated freezing and thawing.

• Stability: Shelf life: one year from despatch.

• Predicted Protein Size: ~ 110 kDa
• Gene Name: androgen receptor
• Database Link:
  Entrez Gene 367 Human
  UniProt ID P10275
• Background: Androgens exhibit a wide range of effects on the development, maintenance and regulation of male phenotype and make reproductive physiology. The androgen receptor (AR) is a member of the steroid superfamily of liganddependent transcription factors. ARs bind the two biologically active androgens, testosterone (T) and dihydrotestosterone (DHT), with high and nearly identical affinities; however, the rates of association and dissociation of T are about three times more rapid than those of DHT. This difference has resulted in speculation as to whether these differences in binding kinetics could account for the different physiological effects of T and DHT. A striking feature of AR is its rapid degradation in the absence of ligand. It is now well established that androgen binding results in an at least six-fold increase in androgen stability and that ligand-induced stabilization of AR is highly androgen- specific.
• Synonyms: Dihydrotestosterone receptor, DHTR, NR3C4